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电话: 400-6721-600 Human BMP-6 ELISA Kit This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK11281 C

48 T EK11282 C

96 T EK11283 C

2 *96 T EK11284 C

5 *96 T EK11285 C

10 *96 T For the quantitative determination of human Bone Morphogenetic Protein

6 (BMP-6) concentration in cell culture supernates, serum and plasma. For research use only. Not for use in diagnostic procedures. Human BMP-6 ELISA Kit 05/2016

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电话: 400-6721-600 Human BMP-6 ELISA Kit Table of Contents Introduction Description

1 Principle of the Assay

1 Limitations of the Procedure

1 General Information Materials Provided

3 Other Supplies required

3 Storage

5 Precaution

7 Technical Hints

9 Assay Protocol Sample Collection and Storage

9 Reagent Preparation 11,13 Assay Procedure

15 Analysis Calculation of Results

17 Typical Data

17 Sensitivity

19 Precision

19 Recovery

21 Linearity

21 Calibration

21 Sample Values

23 Specificity

23 Assay Procedure Summary

25 目录 产品介绍 背景介绍

2 检测原理

2 试剂盒检测的局限

2 基本信息 试剂盒提供的材料

4 未提供的材料设备

4 贮存

6 注意事项

8 技术要点

10 检测步骤 样本的收集和贮存

10 试剂准备 12,

14 检测步骤

16 分析 结果计算

18 典型数据

18 灵敏度

20 精密度

20 回收率

22 稀释线性

22 校准

22 样本值

24 特异性

24 检测步骤概要

26

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电话: 400-6721-600 Human BMP-6 ELISA Kit DESCREPTION Bone morphogenetic protein

6 (BMP-6) encoded by the BMP6 gene is a member of the TGF-β superfamily. Bone morphogenetic proteins are known for their ability to induce the growth of bone and cartilage. BMP-6 is able to induce all osteogenic markers in mesenchymal stem cells, an additional function of BMP-6 has been identified as described in Nature Genetics in 2009. BMP-6 is the key regulator of hepcidin, the small peptide secreted by the liver which is the major regulator of iron metabolism in mammals. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human BMP-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and BMP-6 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for BMP-6 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of BMP-6 bound in the initial step. The color development is stopped and the intensity of the color is measured. LIMITATIONS OF THE KIT FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. The kit should not be used beyond the expiration date on the kit label. Do not mix or substitute reagents with those from other lots or sources. If samples generate values higher than the highest standard, dilute the samples with Assay Buffer and repeat the assay. If cell culture supernate samples require large dilution, perform an intermediate dilution in culture medium. Any variation in standard dilution, operator, pipetting technique, washing techniques, incubation time or temperature, and kit age can cause variation in binding. This assay is designed to eliminate interference by factors present in biological samples. Until all factors have been tested in the ELISA immunoassay, the possibility of interference cannot be excluded. 背景介绍 骨形态发生蛋白