PDF《茯苓摇瓶液体发酵复合药性培养基及其化学成分研究》

中草药 Chinese Traditional and Herbal Drugs 第43 卷第8期2012 年8月・1519・ 茯苓摇瓶液体发酵复合药性培养基及其化学成分研究 李羿1,杨 胜1,李 晨1,杨万清

1 ,万德光 2* 1.

成都医学院药学院,四川 成都

610083 2. 成都中医药大学药学院,四川 成都

610075 摘要:目的 筛选茯苓摇瓶液体发酵最适复合药性培养基,并对复合药性发酵茯苓的化学成分进行研究.方法 在不同复 合药性培养基中进行茯苓摇瓶液体发酵, 测定其胞外多糖和菌丝体产量. 根据复合药性发酵茯苓化学成分的理化性质进行提 取分离和定量测定.结果 在发酵基础培养基中添加 0.75%薏苡仁和枸杞子组成最适复合药性培养基,茯苓菌丝体干质量达 14.29 g/L,茯苓胞外多糖达 8.22 g/L.药性发酵茯苓中总多糖、总糖、总灰分、氨基酸总量分别为 25.35%、45.73%、4.79%、 21.43%.结论 不同种类和不同数量的中药材组成的复合药性培养基对茯苓摇瓶液体发酵有较大影响,为其液体发酵产业 化提供理论依据. 关键词:茯苓;

摇瓶液体发酵;

复合药性培养基;

胞外多糖;

氨基酸 中图分类号:R284.2 文献标志码:A 文章编号:0253 - 2670(2012)08 -

1519 -

04 Compound medicinal medium of flask liquid fermentation of Poria cocos and its chemical constituents LI Yi1 , YANG Sheng1 , LI Chen1 , YANG Wan-qing1 , WAN De-guang2 1. School of Pharmacy, Chengdu Medical College, Chengdu 610083, China 2. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China Abstract: Objective To screen the optimum compound medicinal medium for flask liquid fermentation of Poria cocos and study the chemical constituents in compound medicinal fermented P. cocos. Methods P. cocos was flask liquid fermented in different compound medicinal media and the exocellular polysaccharide and dry weight of mycelium were detected. The chemical constituents were isolated and detected according to the physicochemical properties. Results The optimum medicinal fermentation medium was adding 0.75% Coicis Semen and Lycii Fructus into fermentation basic medium. In the optimum compound medicinal fermentation medium, the dry weight of P. cocos mycelium was 14.29 g/L and the exocellular polysaccharide was 8.22 g/L. The total polysaccharides, total saccharides, total ashes, and total amino acids in compound medicinal fermented P. cocos were 25.35%, 45.73%, 4.79%, and 21.43%, respectively. Conclusion Different kinds and amounts of traditional Chinese medicinal materials (TCMM) in compound medicinal fermentation medium have various effects on flask liquid fermentations of P. cocos. The chemical constituents in compound medicinal fermented P. cocos are studied to provide a theoretical basis for its industrialization of liquid fermentation. Key words: Poria cocos (Schw.) Wolf;

flask liquid fermentation;

compound medicinal fermentation medium;

exocellular polysaccharide;

amino acid 茯苓为多孔菌科真菌茯苓 Poria cocos (Schw.) Wolf 的菌核,有利水渗湿、健脾安神的功效.茯苓 为常用中药材,市场需求量巨大.过度采挖野生茯苓 导致其资源逐渐枯竭;

人工栽培茯苓需消耗大量的松 材,对生态环境造成严重的破坏.因此,亟需寻找野 生茯苓和人工栽培茯苓的代用品. 液体发酵可实现茯 苓的工业化生产,缩短生产时间,节省大量的松材, 对我国植物资源和生物多样性保护意义重大. 现代发 酵工程技术已广泛应用于药用真菌的培养[1-2] ,推动 了其工业化生产和资源的科学开发利用. 收稿日期:2012-04-06 基金项目:四川省教育厅科研基金项目(09ZC031) ;