PDF《DNA 疫苗海藻酸纳微球的制备及体外释药》

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58 ? 第二军医大学学报

2004 Jan;

25 (1) Acad J Sec Mil Med Univ ・抢著・ DNA 疫苗海藻酸纳微球的制备及体外释药 刘善奎高 中l\ 钟延强张洪英手j、树汉2 O.

第二军医大学药学院药剂学教研室,上海 200433;

2. 基础医学部医学遗传学教研室) [摘要] 町的:研制 DNA 疫苗海藻酸纳微球,并对其体外释药特性进行考察.方战:以海藻酸纳为载体,采用 w/o 乳化-离 子交联法制备 DNA 疫苗海藻酸纳微球;

考察粒径大小、外观、载药量等理化特性;

考察微球的体外释药特性及影响因素.结果:微球球形圆整,分散性好,平均粒径为 02.03 土6. 9)μm ,载药量可达 5% ,包封率为 56.0% ;

微球的体外释放速率与载药 量呈正相关,与壳聚糖的交联固化度呈负相关.结抢:海藻酸纳可以作为 DNA 疫苗微球的可生物降解辅料;

乳化离子交联法 的制备工艺简便,有利于 DNA 疫苗结构和功能的稳定性;

海藻酸纳微球是 DNA 疫苗的一种理想给药系统. [关键词] 疫苗, DNA;

海藻酸纳;

微球;

体外释药 [中图分类号] R 392-33 [文献标识码] A C 文章编号] 0258-879X(2004)01-0058-03 Preparation and in vitro release of DNA vaccine loaded sodium alginate microspheres LIU Shan-Kui1 , GAO Shen1 棍,ZHONG Yan-Qiang1 , ZHANG Hong-Ying2 , SUN Shu-Han' (1. Department of Pharmaceu tics , School of Pharmacy , Second Military Medical U niversity , Shanghai

200433 , China;

2. Department of Medical Genetics , College of Basic Medical Sciences) [ABSTRACT] Objective: To prepare DNA vaccine loaded sodium alginate microspheres CDNA-SA-MS) and to evaluate its dissolution characteristics in vitro. Methods: DNA-SA-MS was prepared with sodium alginate as carriers by the water in oil CW /0) emulsion-ion cross-linking method. Physical and chemical characteristics of microspheres , such as mean diameter , morphology and drug loading were detected. The release characteristics in vitro and its influencing factors were also detected. ResuIts;

Microspheres with good shape and dispersity were prepared. The mean diameters , drug loading and drug entrapment efficiency were 02.03 土6. 9)μm , 5% and 56. 0% respectively. Release rate was negatively correlated with cross-linking ex- tent of chitosan , positively correlated with the drug contents. Conclusion: Sodium alginate can be used as biodegradable carri- ers for the preparation of DNA vaccines microspheres and the microspheres can be used as ideal drug delivery system for DNA vaccines. The W /0 emulsion-ion cross-linking method is simple and particularly good for the structural and functional integri- ty of DNA vaccines.