PDF《IL-6 Antibody》

Cat.

No.: 38-201 To detect Human IL-6 by sandwich ELISA (using

100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Human IL-6 (38- 202) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6. To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non- reducing conditions. To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non- reducing conditions. IL-6 Antibody SPECIFICATIONS HOST SPECIES: Goat SPECIES REACTIVITY: Human IMMUNOGEN: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-6. Human IL-6 specific antibody was purified by affinity chromatography employing immobilized hIL-6 matrix. TESTED APPLICATIONS: ELISA, Neut, WB APPLICATIONS: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (5 ng/mL), a concentration of 0.05 - 0.08 ug/mL of this antibody is required. ELISA: Indirect: To detect hIL-6 by indirect ELISA (using

100 uL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Sandwich To detect hIL-6 by sandwich ELISA (using

100 uL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti- Human IL-6 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Western Blot: To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. PROPERTIES CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Lyophilized STORAGE CONDITIONS: IL-6 antibody is stable for at least

2 years from date of receipt at -20?C. The reconstituted antibody is stable for at least two weeks at 2-8?C. Frozen aliquots are stable for at least

6 months when stored at -20?C. Avoid repeated freeze-thaw cycles. ADDITIONAL INFO OFFICIAL SYMBOL: IL6 ALTERNATE NAMES: HGF, HSF, BSF2, IL-6, IFNB2, Interleukin-6, B-cell stimulatory factor

2 ACCESSION NO.: P05231 PROTEIN GI NO.:

124347 GENE ID:

3569 USER NOTE: Centrifuge vial prior to opening. BACKGROUND AND REFERENCES BACKGROUND: Interleukin

6 (IL-6) is a multifunctional

24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL-6 has also been implicated in regulating adipose mass. For additional information, visit ProSci's Terms & Conditions Page. ANTIBODIES FOR RESEARCH USE ONLY. March 30, 2019