PDF《崔文璟【个人简介】_崔文璟》

【个人简介】 姓名崔文Z 性别男出生日期 1982.

7 职称副教授 电话0510-85197551 传真0510-85197551 E-mail wjcuiATjiangnan.edu.cn (替换 AT 为@) 【学术简介】 工作经历 2014.9- 江南大学生物工程学院, 副教授, 硕士生导师 2010.11-2014.10 江南大学生物工程学院, 讲师 2017.1-2018.1 康奈尔大学化学与生物分子工程学院,访问学者 教育经历 2007.9-2010.6 南开大学 生物化学与分子生物学 博士 2005.9-2007.6 吉林大学 生物化学与分子生物学 硕士 2001.9-2005.6 河南农业大学 动物医学 学士 学术任职 Frontiers in Bioengineering and Biotechnology 编委会 Review Editor 美国化学学会会员 【研究领域】 ? 微生物合成生物学.细菌生物元件的发掘、改造、标准化,人工合成遗传途径、基因线路的设计;

蛋白质和活性多肽的生物合成. ? 蛋白质工程和酶工程.利用蛋白质工程手段改造工业和医药用酶,如纳豆激酶、腈水合酶、天冬 氨酸-α-脱羧酶等,用于工业催化生产平台化学品和生物转化制备药物中间体. 【主要论著】 (著作和论文) 近几年 SCI 论文 1) Laichuang Han, Feiya Suo, Cui Jiang, Jie Gu, Ningna Li, Naixin Zhang, Wenjing Cui*, Zhemin Zhou*. Fabrication and characterization of a robust and strong bacterial promoter from semi-rationally engineered promoter library in Bacillus subtilis. Process Biochem. 2017, Jun (in press) 2) Wenjing Cui, Jintao Cheng, Shengnan Miao, Li Zhou, Zhongmei Liu, Junling Guo, Zhemin Zhou*. Comprehensive characterization of a theophylline riboswitch reveals two pivotal features of Shine-Dalgarno influencing activated translation property. Appl Microb Biotechnol. 2017, 101(5):2107-2120 3) Wenjing Cui, Laichuang Han, Jintao Cheng, Zhongmei Liu, Li Zhou, Junling Guo, Zhemin Zhou * . Engineering an inducible gene expression system for Bacillus subtilis from a strong constitutive promoter and a theophylline-activated synthetic riboswitch. Microb Cell Fact. 2016, 15:199. 4) Chengran Guan?, Wenjing Cui*?, Jintao Cheng, Li Zhou, Zhongmei Liu, Zhemin Zhou*. Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis. Microb Cell Fact. 2016, 15(1):66. 5) Chengran Guan, Wenjing Cui, Jintao Cheng, Rui Liu, Zhongmei Liu, Li Zhou, Zhemin Zhou*. Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis. N Biotechnol. 2016, 33(3):372-379. 6) Zhongyi Cheng ?, Wenjing Cui ?, Zhongmei Liu, Li Zhou, Min Wang, Michihiko Kobayash*, Zhemin Zhou*. A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards alpha,omega-dinitriles. Catal Sci Technol. 2016, 6(5):1292-1296. 7) Jintao Cheng, Chengran Guan, Wenjing Cui, Li Zhou, Zhongmei Liu, Li Weijiang*, Zhemin Zhou*. Enhancement of a high efficient autoinducible expression system in Bacillus subtilis by promoter engineering. Protein Expr Purif. 2016, 127:81-87. 8) Yuanyuan Xia, Wenjing Cui, Zhongmei Liu, Li Zhou, Youtian Cui, Michihiko Kobayashi*, Zhemin Zhou*. Construction of a subunit-fusion nitrile hydratase and discovery of an innovative metal ion transfer pattern. Sci Rep. 2016, 6:19183. 9) Li Zhou, Wenjing Cui, Zhongmei Liu, Zhemin Zhou*. Metabolic engineering strategies for D-lactate over production in Escherichia coli. J Chem Technol Biot. 2016, 91(3):576-584. 10) Chengran Guan, Wenjing Cui*, Jiantao Cheng, Li Zhou, Junling Guo, Xu Hu, Guoping Xiao, Zhemin Zhou*. Construction and development of an auto-regulatory gene expression system in Bacillus subtilis. Microb Cell Fact. 2015, 14(1):150. 11) Chengran Guan, Wenjing Cui?, Xiaotian He, Xu Hu, Guocheng Du, Jian Chen, Zhemin Zhou*. Construction and development of a novel expression system of Streptomyces. Protein Expr Purif. 2015, 113:17-22. 12) Wenjing Cui?, Zengxiu Shi ?, Yueqin Fang, Li Zhou, Ning Ding, Zhemin Zhou* . Significance of Arg3, Arg54, and Tyr58 of L-aspartate α-decarboxylase from Corynebacterium glutamicum in the process of self-cleavage. Biotechnol Lett, 2014, 36(1):121-126. 13) Youtian Cui, Wenjing Cui, Zhongmei Liu, Li Zhou, Michihiko Kobayashi, Zhemin Zhou* . Improvement of stability of nitrile hydratase via protein fragment swapping. Biochem Bioph Res Comm. 2014, 450: 401-408. 14) Wenjing Cui, Shuai Zhang, Changliang Shan, Li Zhou, Zhemin Zhou* . microRNA-133a regulates cell cycle and proliferation of breast cancer cells by targeting Epidermal Growth Factor Receptor through EGFR/Akt signaling pathway. FEBS J, 2013, 280(16):3962-3974. 15) Wenjing Cui, Xiaoyan Yang, Yueqin Fang, Shengmin Zhou, Song Liu, Guocheng Du, Kun Du, Jian Chen, Guanjun Tao, Zhemin Zhou* . Discovery of two transglutaminases derived from same zymogen from Streptomyces hygroscopicus and analysis for the formation processes. J Sci Food Agric, 2013, 93(7):1711-1717. 16) Wenjing Cui, Yu Zhao, Changliang Shan, Guangyao Kong, Nan Hu, Yingyi Zhang, Shuai Zhang, Weiying, Zhang, Xiaodong Zhang* , and Lihong Ye* . HBXIP upregulates CD46, CD55 and CD59 through ERK1/2/NF-kappaB signaling to protect breast cancer cells from complement attack. FEBS Lett, 2012, 586(6): 766-771. 著作 《酶工程原理与技术》(第三版),参编,高等教育出版社,北京;