PDF《低功率2 450 MHz 射频磁场对神经元 延迟整流钾通道的抑制作...》

ISSN 1007-

7626 CN 11-

3870 /Q 中国生物化学与分子生物学报 http: / /cjbmb.

bjmu. edu. cn Chinese Journal of Biochemistry and Molecular Biology

2012 年6月28( 6) :

561 ~

566 收稿日期: 2012- 01- 21;

接受日期: 2012- 04-

09 天津市自然科学基金( No. 08JCYBJC14700) * 联系人 Tel: 13820201015;

E - mail: zhengyu@ tjpu. edu. cn Received: January 21,2012;

Accepted: April 9,2012 Supported by National Natural Science Foundation of Tianjin ( No. 08JCYBJC14700) * Corresponding author Tel: 13820201015;

E-mail: zhengyu@ tjpu. edu. cn 低功率2

450 MHz 射频磁场对神经元 延迟整流钾通道的抑制作用 郑羽1) , 2) * , 张洋1) , 张子钊 1) , 王金海 1) , 李刚2) , 程立君 3) ( 1) 天津工业大学电子与信息工程学院, 天津 300387;

2) 天津大学精密测试技术及仪器国家重点实验室, 天津 300072;

3) 天津市医科大学第二附属医院天津心脏病学研究所, 天津 300211) 摘要 由于电子设备的广泛使用, 人类已经越来越多地暴露在射频磁场的辐射下, 但射频磁场的辐 射效应却一直不明确. 采用全细胞膜片钳技术, 记录2

450 MHz 射频磁场辐射对小鼠脑皮层神经元 延迟整流钾电流 IK 的影响. 利用 Ansoft HFSS 软件对

6 dB 全向天线建模仿真, 验证距天线

2 ~

3 cm 处磁场分布均匀, 使用 Agilent E5070B 网络分析仪经该天线发射出2

450 MHz 输出功率为 39.

81 mW 电磁场, 对细胞进行刺激. 实验发现,

2 450 MHz 低功率射频磁场暴露

5、

10 和15 min 对IK 均有 明显的抑制作用;

显著影响IK 激活特性, 对照组与磁场暴露组半数激活电压分别为(-1.

13 ± 2. 32) mV 和( 19.

52 ± 1. 03) mV( n = 10,P <

0. 05) ;

斜率因子分别为( 23.

21 ± 3. 29) mV 和( 13.

95 ± 1. 27) mV( n = 10,P <

0. 05) . 结果表明, 低功率射频磁场通过减小延迟整流钾通道电流, 影响神 经元的生理功能, 为进一步研究电磁辐射所引发的生物学效应提供了一种新的方法. 关键词 延迟整流钾电流;

2 450 MHz 射频磁场;

皮层神经元 中图分类号 Q6 -

3 Inhibitory Effects of Neuron Delayed Rectifier Potassium Channel under Low Power

2 450 MHz RF Magnetic Field ZHENG Yu1) , 2) * ,ZHANG Yang1) ,ZHANG Zi-Zhao1) ,WANG Jin-Hai1) , LI Gang2) ,CHENG Li-Jun3) ( 1) School of Electronics and Information Engineering, Tianjin Polytechnic University,Tianjin 300387, China;

2) State Key Laboratory of Precision Measurement Technology and Instruments,Tianjin University,Tianjin 300072,China;

3) Institute of Cardiology of Tianjin,The Second Hospital of Tianjin Medical University,Tianjin 300211, China) Abstract With the wide applications of electronic devices,people are increasingly exposed on radio frequency ( RF) magnetic field. However,the effects of RF magnetic field on people'

s health remained unclear. Delayed rectifier potassium current ( IK ) was studied in isolated rat neurons which were exposed to

2450 MHz radio frequency ( RF) magnetic field via whole cell patch clamp technique. Ansoft HFSS was used to simulate

6 db gain omni directional antenna magnetic field distribution. The result showed that magnetic field density uniform distribution where

2 ~

3 cm far from the antenna. The

2 450 MHz RF magnetic field was sent out by a

6 dB gain omni directional antenna attached on an Agilent E5070B net analyzer and the transmitting power was 39.

81 mw. Compared to the control group, a significant inhibition on the IK after exposure to low power