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tandfonline.com/action/journalInformation?journalCode=tejp20 British Phycological Journal ISSN: 0007-1617 (Print) (Online) Journal homepage: https://www.tandfonline.com/loi/tejp19 A simple separation technique for purifying micro- algae S.I. Heaney &

G.H.M. Jaworski To cite this article: S.I. Heaney &

G.H.M. Jaworski (1977) A simple separation technique for purifying micro-algae, British Phycological Journal, 12:2, 171-174, DOI: 10.1080/00071617700650191 To link to this article: https://doi.org/10.1080/00071617700650191 Published online:

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1 June

1977 A SIMPLE SEPARATION TECHNIQUE FOR PURIFYING MICRO-ALGAE By S. I. HEANEYand G. H. M. JAWORSKI Freshwater Biological Association, The Ferry House, Ambleside, Cumbria LA22 0LP A simple separation technique using polycarbonate membrane filters for purifying micro- algae is described. The mechanical separation of algal cells from other organisms is generally regarded as the most satisfactory method of obtaining pure cultures of algae. Techniques based on this principle have been described by several workers (e.g. Allen, 1952;

Droop, 1969;

McCurdy &

Hodgson, 1974) and have included the separation of algae and bacteria by filtration. Recent advances in membrane filter technology have made possible the efficient separation of particles of different sizes and enabled simple procedures for the purification of algal cultures to be developed. This work describes the use of polycarbonate mem- brane filters, which have advantages over conventional cellulosic membranes, to isolate pure clonal cultures of two important planktonic algae. METHODS THE SEPARATION OF ALGAE FROM BACTERIA The apparatus shown in Fig.

1 was used to separate the algae from other smaller organisms in culture. It consists of a

25 mm diameter Nucleopore Swin-LokTM membrane holder (A) (Nucleopore Corporation, Pleasanton, California, U.S.A.) containing an 8.0/~m maximum pore diameter Nucleopore membrane. This pore size was chosen as it retained most of the algae but allowed the bacteria to pass through. A glass syringe and silicon rubber plug were attached to the cap of the membrane holder and a silicon rubber tube attached to its base. The tube was connected to a Buchner flask and a vacuum provided by a small pump. A

13 mm diameter membrane holder (B) containing a

0 22 tzm pore size membrane was attached to the effluent tube via a T piece. This filter was connected to the laboratory air supply and sterilized the air used for backwashing;

the filter was raised slightly from the horizontal to prevent culture medium entering. Two clamps (C and D) were attached to the tubing as indicated, and these allowed the vacuum and air pressures to be varied. Sterile culture medium was added to the syringe with the clamps C and D closed. The vacuum pump and air supply were switched on and the clamps adjusted so that the culture medium was drawn slowly through the membrane filter. With further careful adjustment of these clamps, it was possible to achieve conditions where the liquid was alternately drawn through and backwashed across the membrane. The optimal adjustments were when slightly more medium was drawn from the syringe than was backwashed into it. When the apparatus was working satisfactorily about

50 ml of culture medium was filtered through followed by a few mls of algal culture (culture density c.