PDF《Anti-BMP4》

Anti-BMP4 produced in rabbit, affinity isolated antibody Catalog Number SAB4200556 Product Description Anti-BMP4 is produced in rabbit using as immunogen a synthetic peptide corresponding to an internal sequence of human BMP4 (GeneID: 652), conjugated to KLH.

The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse BMP4. The antibody is affinity-purified using the immunizing peptide immobilized on agarose. Anti-BMP4 specifically recognizes human BMP4. The antibody may be used in several immunochemical techniques including immunoblotting (~55 kDa), immunoprecipitation and immunohistochemistry. Detection of the BMP4 band by immunoblotting is specifically inhibited by the BMP4 immunizing peptide. Bone morphogenetic proteins (BMPs) are members of the TGF-β superfamily. BMPs were originally identified as growth factors that can induce bone and cartilage formation.1,2 More than

30 members of the BMPs family have been identified which are involved in a large variety of functions during development and in cancer. BMPs are synthesized as precursor molecules, followed by enzymatic cleavage and release as active ligands. BMPs exert their biological function by binding to Ser/Thr kinase receptors including ALK2, BMPR-IA/ALK3, BMPR-IB/ALK6 and BMPR-II, and to phosphorylated Smads, mainly Smad1, Smad5 and Smad8.2 BMP4 (known as ZYME, BMP2B) regulates cell proliferation, differentiation, apoptosis and cell fate determination during development. Specifically, BMP4 has been shown to regulate mesodermal cell commitment to the hematopoietic cell lineage during early embryogenesis.

3 BMPs are expressed in localized patterns during embryonic mouse brain development.1 BMP4 is crucial for development since knockout mice die early in embryogenesis. Overexpression of BMP4 has been reported in several cancers, including melanoma, hepatocellular carcinoma, adenocarcinoma and colorectal cancer and is frequently accompanied by an enhanced cell migratory and invasive potential of the tumors.2,4-6 Reagent Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing

15 mM sodium azide. Antibody Concentration: ~1.0 mg/mL Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Storage/Stability For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within

12 hours. Product Profile Immunoblotting: a working concentration of 1-2 ?g/mL is recommended using extracts of CHO cells over- expressing human BMP4. Immunoprecipitation: a working amount of

10 ?g is recommended using lysates of CHO cells over- expressing human BMP4. Immunohistochemistry: a working concentration of

20 ?g/mL is recommended using formalin-fixed, paraffin-embedded human colon. Note: In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration. References 1. Mehler, M.F., et al., Trends Neurosci., 20, 309-317 (1997). 2. Hardwick, J.C., et al., Nature Rev. Cancer, 8, 806-

812 (2008). 3. Goldman, D.C., et al., Blood, 114, 4393-4401 (2009). 4. Rothhammer, T., et al., Cancer Res., 65, 448-456 (2005). 5. Deng, H., et al., Exp. Cell Res., 313, 1033-1044 (2007). 6. Maegdefrau, U., et al., J. Pathol., 218, 520-529 (2009). ER,KCP,PHC 01/13-1 ?2012 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip.