PDF《杜仲幼杜仲 MEP 途径系列基因表达差异的研究》

42 Unigene were annotated by MEP pathway, of them, the expression of DXS2 and DXS3 were significantly different between young fruit and leaf, and the expression of DXS2 was the highest;

7 Unigenes of DXS were annotated in young fruit and mature fruit., of them, DXS6 only was expressed in fruit and the expressions of DXS2, DXS3, DXS5 and DXS7 were with significant differences.

4 Unigenes of DXR were annotated in young fruit and leaf. The expression of DXR

1、DXR

2、DXR3 were significantly different.3 Unigenes were annotated in young fruit and fruit.DXR3 only expressed in fruit.Expression of all Unigenes were not significantly different.Expression of DXR1 was highest.Only

1 Unigene of MCT was annotated in young fruit and leaf and it'

s expression was significantly different.Only

1 Unigene was annotated in young fruit and fruit and it'

s expression was not significantly different. Only

1 Unigene of CMK was annotated in young fruit and leaf and it'

s expression was not significantly different.Only

1 Unigene was annotated in young fruit and fruit and it'

s expression was not significantly different.Only

1 Unigene of MDS was annotated in young fruit and leaf and it'

s expression was significantly different.2 Unigenes were annotated in young fruit and fruit and it'

s expression was not significantly different.Only

1 Unigene of HDS was annotated in young fruit and leaf and it'

s expression was significantly different. Only

1 Unigene was annotated in young fruit and fruit and it'

s expression was significantly different.5 Unigenes of HDR were annotated in young fruit and leaf.Expression of HDR1and HDR5 were significantly different.While

7 Unigenes were annotated in young fruit and

27 第34 卷中南林业科技大学学报杜仲 Eucommia ulmoides 为杜仲科杜仲属多年 生落叶乔木,杜仲科仅

1 属1种,中国是现存杜 仲的唯一原产地 [1-2] ,有学者在北美发现了杜仲的 化石 [3] ,来自美国、加拿大和墨西哥的杜仲的

25 个生长地的植物残骸表明该物种曾经在新生代广 泛分布与北美的各个地方.杜仲是中国特有的名 贵经济树种,也是世界适应范围最广的重要胶源 植物 [4] .杜仲以干燥的根皮入药,具有补肝肾、强 筋骨、降血压、安胎等诸多功效,用于治疗肾虚 腰疼、筋骨无力、妊娠漏血、胎动不安等多种疾 病[5] .Jin Nyoung HO[6] 等人于

2005 年的研究了从 杜仲中提取的桃叶珊瑚苷在紫外线 -B(UVB)诱 导的人类成纤维细胞的氧化应激反应中所起到的 保护作用,研究发现经过 UVB 的照射后,用桃叶 珊瑚苷预处理的人体皮肤成纤维细胞 (Hs68株系) , 能够明显抑制 57% 的基质金属蛋白酶 -1(MMP- 1)的产生.杜仲胶是世界极具发展潜力的优质天 然橡胶资源,Shinya Takeno[7] 等人于

2008 年利用 傅里叶变换红外光谱和裂解气象色谱 / 质谱来定 量分析了杜仲中的反式 -1,4- 聚异戊二烯,并成 功地将提取 / 定量的方法应用于研究杜仲橡胶含 量和分子量的季节性变化,FT-IR 分析表明叶子中 的杜仲胶随着季节的变化是逐渐积累的过程,从SEC 分析得到的数据看来随着季节变化低分子量 的橡胶(Mn=6.0*103 )所占的比重呈增加趋势. Nobuaki Suzuki[8] 等人于

2012 年构建并分析了反式 聚异戊二烯树种杜仲 EST 文库,该研究构建了一 个杜仲茎外周组织与内周组织的 cDNA 文库,包含27752 个表达序列标签(ESTs),组装成