PDF《proGDNF Antibody - Background》

9765 Clairemont Mesa Blvd, Suite C San Diego, CA

92124 Tel: 858.

875.1900 Fax: 858.622.0609 proGDNF Antibody Affinity purified polyclonal antibody Catalog # AG1167 Specification proGDNF Antibody - Product Information Application WB Primary Accession P39905 Reactivity Human, Mouse, Rat Host Rabbit Clonality Polyclonal Calculated MW

23720 Homology Rat, mouse - identical. proGDNF Antibody - Additional Information Gene ID

2668 Other Names Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF Related products for control experiments Control peptide antigen (supplied with the antibody free of charge). Target/Specificity Peptide (C)PEDYPDQFDDVMD, corresponding to amino acid residues 54-66 of human GDNF (precursor) (Accession P39905). Pro-domain of the GDNF protein.The antibody is specific for the pro-domain region of GDNF and will not react with mature GDNF. Dilution WB~~1:200-1:2000 Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis. Format Affinity purified antibody, lyophilized powder Reconstitution

50 ?l or 0.2 ml deionized water, depending on the sample size. Antibody Concentration After Reconstitution 0.8 mg/ml. Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% Western blot analysis of human GDNF pro-domain fusion protein at

40 ng (lanes

1 and 4),

30 ng (lanes

2 and 5) and 20ng (lanes

3 and 6): 1-3. Anti-proGDNF antibody (#AG1167), (1:200). 4-6. Anti-proGDNF antibody, preincubated with the control peptide antigen. Note that the expected MW of the GDNF pro-domain fusion protein is 21kD proGDNF Antibody - Background Glial-Derived Neurotrophic Factor (GDNF) is a member of the TGF-β superfamily. GDNF signals through a multi-component receptor system, composed of a RET protooncogene and one of the four α1-α4 receptors1. GDNF promotes survival of various neuronal cells, including motoneurons2,3, Purkinje cells and sympathetic neurons4. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake5. Cells that express GDNF include Sertoli cells, type

1 astrocytes, Schwann cells6, neurons, pinealocytes, and skeletal muscle cells7. In vivo, following transection of facial motor neuron axons, locally applied GDNF has been shown to rescue virtually all damaged neurons from death8. GDNF may be of clinical relevance in the treatment of Parkinson's disease that is characterized by progressive degeneration of midbrain dopaminergic neurons9,10. Recently, it has been hypothesized that functional, carboxy-terminally amidated peptides BSA, 0.05% NaN3. Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Storage After Reconstitution The reconstituted solution can be stored at 4?C for up to

2 weeks. For longer periods, small aliquots should be stored at -20?C or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 * g

5 min). Control Antigen Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Control Antigen Reconstitution

100 ?l DDW. Control Antigen Storage After Reconstitution -20?C. Preadsorption Control

1 ?g peptide per

1 ?g antibody. proGDNF Antibody - Protocols Provided below are standard protocols that you may find useful for product applications. ? Western Blot ? Blocking Peptides ? Dot Blot ? Immunohistochemistry ? Immunofluorescence ? Immunoprecipitation ? Flow Cytomety ? Cell Culture are processed from the GDNF precursor upon proteolytic cleavage by furin-like endopeptidase11,12,13. Those different peptides (a 5-mer and 11-mer) have not been isolated endogenously to date. However, the rat 11-mer sequence (named brain excitatory peptide, BEP) significantly induced synaptic excitability and possessed some dopaminergic activities in vitro (thus named dopamine neuron stimulating peptides, DNSP)13. Furthermore, the human 11-mer sequence (named DNSP-11) exhibits neurotrophic-like properties13. Thus, the role of the full proDomain of GDNF, which is a product of proteolytic cleavage of proGDNF, is not clearly understood yet. Abgent is pleased to offer a highly specific antibody directed against the prodomain of human GDNF. Anti-proGDNF antibody (#AG1167) can be used in Western blot analysis. It has been designed to recognize proGDNF from human, mouse and rat samples. proGDNF Antibody - References References 1. Airaksinen, M.S. et al. (2002) Nat. Rev. Neurosci. 3, 383. 2. Henderson, C.E. et al. (1994) Science 266, 1062. 3. Houenou, L.J. et al. (1996) Cell. Tissue Res. 286, 219. 4. Kobayashi, M. et al. (2000) Neuroreport 11, 2541. 5. Tomac, A. et al. (1995) Nature 373, 335. 6. Zhou, F.Q. et al. (2003) Cell 113, 814. 7. Lin, L.F. et al. (1993) Science 260, 1130. 8. Matheson, C.R. et al. (1997) Neuroreport 8, 1739. 9. Brundin, P. (2002) Brain 125, 2149. 10. Grondin, R. et al. (1998) J. Neurol. 245, P35. 11. Bradley, L.H. et al. (2010) PLoS ONE 5, e9752. 12. Immonen, T. et al. (2008) Exp. Neurol. 210, 793. 13. Kelps, K.A. et al. (2011) Neuropeptides 45, 213. Powered by TCPDF (www.tcpdf.org)