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电话:400-6721-600 Human Growth Hormone/GH ELISA Kit This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK11041 C

48 T EK11042 C

96 T EK11043 C

2 *96 T EK11044 C

5 *96 T EK11045 C

10 *96 T For the quantitative determination of human Growth Hormone (GH) concentration in cell culture supernates, serum and plasma. For research use only. Not for use in diagnostic procedures. Human Growth Hormone/GH ELISA Kit 05/2016

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电话:400-6721-600 Human Growth Hormone/GH ELISA Kit Table of Contents Introduction Description

1 Principle of the Assay

1 Limitations of the Procedure

1 General Information Materials Provided

3 Other Supplies required

3 Storage

5 Precaution

7 Technical Hints

9 Assay Protocol Sample Collection and Storage

9 Reagent Preparation 11,13 Assay Procedure

15 Analysis Calculation of Results

17 Typical Data

17 Sensitivity

19 Precision

19 Recovery

21 Linearity

21 Calibration

21 Sample Values

23 Specificity

23 Assay Procedure Summary

25 目录 产品介绍 背景介绍

2 检测原理

2 试剂盒检测的局限

2 基本信息 试剂盒提供的材料

4 未提供的材料设备

4 贮存

6 注意事项

8 技术要点

10 检测步骤 样本的收集和贮存

10 试剂准备 12,

14 检测步骤

16 分析 结果计算

18 典型数据

18 灵敏度

20 精密度

20 回收率

22 稀释线性

22 校准

22 样本值

24 特异性

24 检测步骤概要

26

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电话:400-6721-600 Human Growth Hormone/GH ELISA Kit DESCREPTION Growth hormone (GH), also known as somatotropin, is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. It is thus important in human development. GH is a stress hormone that raises the concentration of glucose and free fatty acids. It also stimulates production of IGF-1. The most common disease of GH excess is a pituitary tumor composed of somatotroph cells of the anterior pituitary. The effects of GH deficiency vary depending on the age at which they occur. In children, growth failure and short stature are the major manifestations of GH deficiency, with common causes including genetic conditions and congenital malformations. It can also cause delayed sexual maturity. In adults, deficiency is rare, with the most common cause a pituitary adenoma, and others including a continuation of a childhood problem, other structural lesions or trauma, and very rarely idiopathic GHD. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Growth Hormone has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and Growth Hormone present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for Growth Hormone is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Growth Hormone bound in the initial step. The color development is stopped and the intensity of the color is measured. LIMITATIONS OF THE KIT FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. The kit should not be used beyond the expiration date on the kit label. Do not mix or substitute reagents with those from other lots or sources. If samples generate values higher than the highest standard, dilute the samples with Assay Buffer and repeat the assay. If cell culture supernate samples require large dilution, perform an intermediate dilution in culture medium. Any variation in standard dilution, operator, pipetting technique, washing techniques, incubation time or temperature, and kit age can cause variation in binding. This assay is designed to eliminate interference by factors present in biological samples. Until all factors have been tested in the ELISA immunoassay, the possibility of interference cannot be excluded. 背景介绍 在人和其它动物中,生长激素 (GH)可刺激生长、细胞繁殖和细胞再生.因此它对人体发育很 重要. 生长激素是一种应激激素, 可提高葡萄糖和游离脂肪酸的浓度. 它还能促进 IGF-1 的产生. 生长激素过剩最常见的疾病是垂体瘤.生长激素缺失产生的影响因年龄而异.对于小孩,生长停 滞和身材矮小是生长激素缺失的主要表现,通常是由于遗传因素和先天畸形.它也能导致性成熟 推迟.在成年人中,生长激素缺失是很少见的,最常见的原因是脑垂体瘤,其它原因包括儿童时 期生长激素缺失的延续、其它的结构损伤或创伤以及极其罕见的特发性生长激素缺乏症 (GHD). 检测原理 本试剂盒采用双抗体夹心酶联免疫吸附检测技术. 特异性抗人 Growth Hormone 单克隆抗体预 包被在高亲和力的酶标板上. 酶标板孔中加入标准品和待测样本, 经过孵育, 样本中存在的 Growth Hormone 与固相抗体结合.洗涤去除未结合的物质后,加入生物素化的检测抗体孵育.洗涤去除 未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP).洗涤后, 加入显色底物 TMB,避光显色.颜色反应的深浅与样本中 Growth Hormone 的浓度成正比.加入 终止液终止反应,在450 nm 波长(参考波长