PDF《吴志刚二甲苯对锦鲤鱼肝脏抗氧化酶的影响_吴志刚》

生物灾害科学2012, 35(3): 295-299 http://xuebao.

jxau.edu.cn Biological Disaster Science, Vol. 35, No. 3,

2012 [email protected] 收稿日期:2012-09-15 基金项目:国家环保部公益课题(201109004) 、河北省科技厅(12276708D) 、河北省教育厅重点课题(ZH2011119)和河 北省重点学科建设基金项目资助. 作者简介:吴志刚,男,江西婺源人,硕士,E-mail: [email protected];

* 通信作者:沈洪艳,副教授,博士后, 主要从事环境生态研究,E-mail: [email protected]. DOI:10.3969/j.issn.2095-3704.2012.03.016 二甲苯对锦鲤鱼肝脏抗氧化酶的影响 吴志刚,沈洪艳* ,武晨虹,王丽新,张国霞 (河北科技大学 环境科学与工程学院,河北 石家庄 050080) 摘要:采用暴露试验方法,研究了不同浓度二甲苯对锦鲤鱼肝脏组织中超氧化物歧化酶和过氧化氢酶的影响.结 果表明,不同浓度组二甲苯在暴露期间对锦鲤鱼肝脏 SOD 活性的影响较小,只有在个别浓度和个别时间段下发生 了显著诱导或抑制作用;

锦鲤鱼暴露于二甲苯时对 CAT 活性影响主要表现为诱导作用,但CAT 对1/16 96hLC50 浓度二甲苯反应较滞后;

随着暴露时间的延长,试验

15 d 后,CAT 活性随着暴露浓度的降低而整体呈下降趋势;

试验中 CAT 活性的变化与 SOD 活性的变化具有一定的同步性:在3d和15 d 时, 1/4 96hLC50 浓度组的 SOD 活性 受到诱导,CAT 活性也受到诱导;

在12 d 时,1/8 96hLC50 浓度组的 SOD 活性受到抑制,CAT 活性也受到抑制. 在本次暴露试验中,CAT 活性对二甲苯较敏感,从分子水平上反映了污染物对鱼体肝细胞的损伤,也反映了环境 中氧化作用的存在,因此,可以考虑其作为水环境中二甲苯早期污染的生物监测指标. 关键词:二甲苯;

锦鲤鱼;

抗氧化酶;

超氧化物歧化酶;

过氧化氢酶 中图分类号:X174 文献标志码:A 文章编号:2095-3704(2012)03-0295-05 Effects of Xylene on Antioxidant Enzymes in the Liver of Cyprinus carpio WU Zhi-gang, SHEN Hong-yan* , WU Chen-hong, WANG Li-xin, ZHANG Guo-xia (College of Environmental Science and Engineering, Hebei Science and Technology University, Shijiazhuang 050018, China) Abstract: An exposure experiment was conducted to study the effect of different concentrations of xylene on the activity of the antioxidant enzymes: superoxide dismutase (SOD) and catalase (CAT) in liver tissue of Cyprinus carpio. The results showed that it has not significant effect on the activity of SOD in the liver of Cyprinus carpio exposed to xylene at different concentrations. Only in the individual concentration and individual time, the activity of SOD was significantly induced or inhibited. The activity of CAT in the liver of Cyprinus carpio exposed to xylene was mainly induced, but in the concentration of xylene for 1/16 96hLC50 conditions, the reaction of the activity of CAT was slowly. However, with the increased of the exposure time, after

15 days, the activity of CAT, declined with the lower of the exposure concentrations. During the whole exposure experiment, the activity of SOD and CAT changed with synchronization. In the exposure of

3 days and

15 days, the activity of SOD was induced in the concentration of 1/4 96h LC50, the activity of CAT was also induced under the same conditions. In the exposure of

12 days, the activity of SOD was inhibited in the concentration of 1/8 96hLC50, the ・296・ 生物灾害科学第35 卷第

3 期activity of CAT was also inhibited under the same conditions. In this exposure experiment, the activity of CAT was more sensitive to xylene, it reflected the existence of oxidation in the environment, it could be considered as the early pollution of biological monitoring index in the water environment. Key words: Xylene;