DOC《南京林业大学》

南京林业大学 本科毕业设计(论文) 题目: 亚洲百合'

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45SrDNA FISH分析 学院:南方学院 专业:生物技术 学号:n080108121 学生姓名:薛承指导教师:席梦利 职称:副教授 二O一二年五月十二日 亚洲百合'

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45S rDNA FISH分析 摘要:在植物基因组中通常有几百个45S rDNA以串联的方式分布于核仁组织区,而且在不同物种中其数量和位置不同,这为研究同属植物的核型差异提供了一条很好的途径.

本文旨在根据45S rDNA在百合'

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基因组中的数量和位置探讨其染色体的特征.采用荧光原位杂交技术,以45SrDNA基因为探针,对亚洲百合'

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进行了分析,实验结果表明,'

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有14个45S rDNA杂交位点,分别位于第

1、

2、

4、

5、

6、

8、11号染色体上.第

1、2号染色体各有两条分别在短臂近着丝点处、短臂区域有信号位点;

第

5、8号染色体各有一条染色体在长臂区域有信号位点,5号染色体的另一条染色体在着丝点区域和长臂末端有信号位点;

第

4、6号染色体各有一条在长臂近着丝点区域有信号点,4号染色体另一条在长臂区域也有信号点,6号染色体另一条则在长臂末端有杂交位点;

第11号染色体其中一条在着丝点区域有信号位点,另一条则在端部区域有信号点.其中4号染色体的两条染色体的杂交位点与5号染色体长臂区域的杂交位点荧光强度偏弱,其他信号点荧光强度基本无差异. 关键字:百合;

45SrDNA;

荧光原位杂交 45S rDNA FISH analysis of Asian Lily '

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Abstract: In plant genomes usually have hundreds of 45S rDNA in tandem located in the nucleolar organizer region, and the number and location in different species, the karyotype differences belong to the same plant provides a goodways. This paper aims to investigate the chromosome characteristics according to the number and location of 45S rDNA Lily '

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genome. Using fluorescence in situ hybridization to 45SrDNA gene as a probe of Asiatic Lily '

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analysis, experimental results show that the '

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14 45S rDNA hybridization sites are located at 1, 2,

4 , 5,6,8,11 chromosome. 1, 2, each chromosome two, respectively, in the short arm near the centromere region of the short arm of the signal;

signal of the first 5,8 chromosome each chromosome long arm region, on chromosome

5 another one chromosome in the centromere region and the long arm terminal signal;

4,6 chromosomes each with a long arm near the centromere region of signal points, another on chromosome

4 in the long arm of the regional signal points, one on chromosome

6 in the long arm terminal hybridization sites;

on the 11th chromosome one in the region of the centromere signal, another signal point in the end zone. The fluorescence intensity of chromosome 4, two chromosome hybridization sites and the hybridization sites of the long arm of chromosome

5 region is weak, the fluorescence intensity of signal point is basically no difference. Key words: Lily;

45 SrDNA;

Fluorescence in situ hybridization 目录1文献述综

3 1.1百合的概况

3 1.2百合的细胞遗传学研究进展

3 1.2.1 百合的核型研究进展

3 1.2.2 百合的Giemsa C-分带研究进展

4 1.2.3 百合的荧光原位杂交研究进展

5 1.3荧光原位杂交

5 1.3.1 荧光原位杂交技术的发展