PDF《如何才能更安全更有效的防控PRRS》

9 1 PRRS灭活疫苗免疫攻毒试验 Immune challenge test with PRRS inactivated vaccine PRRS灭活疫苗田间试验 Field trial of PRRS inactivated vaccine PRRS防控的挑战 The significance of PRRS prevention and control 结论Conclusion

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4 内容提要 Abstract 佑本疫苗 至纯至美 2018/12/4 ZFX版权

10 试验目的: The purpose of the test: 1. 单独使用灭活疫苗的免疫攻毒效果观察;

Observe the effect of immune challenge test on the use of inactivated vaccine alone. 2. 不同免疫组免疫攻毒效果观察. Observe the effect of immune challenge test of different immunization groups. PRRS灭活疫苗(佑蓝宝)免疫攻毒试验(1) PRRS inactivated vaccine(You Lanbao) immune challenge test(1) 佑本疫苗 至纯至美 2018/12/4 ZFX版权

11 1.1 试验设计Test design 试验分组 Grouping 免疫Immunization 攻毒Challenge 序号 NO. 名称Name 数量 Quantity 免疫剂量(每头) IMD(per head) 免疫日龄 Immune day age 攻毒时间 time 攻毒剂量 dose

1 转移因子+3倍灭 活抗原 Transfer factor+3 times inactivated antigen

11 提前注射:转移因子2ml 2ml transfer factor 一免:灭活苗2ml3倍灭活抗原 First immunity:2ml inactivated vaccines(3 times inactivated antigen ) 转移因子:其它组首免第11 天Transfer factor:11th day after first immunity in other groups 一免:其他组二免时First immunity:second immunity in other groups 二免后28天28 days after second immunity 第4代血毒,(106.4 TCID50/ml).PBS 10倍 稀释,每组按1ml、3ml 两个攻毒剂量分别注射 高于国标2.5倍The 4th generation blood virus(106.4 TCID50/ml) was diluted by

10 times and is injected 1ml and 3ml respectively in each group. 2.5 times than the national standard.

2 3倍灭活抗原+3 倍灭活抗原

3 times inactivated antigen +3 times inactivated antigen

11 首免:灭活苗2ml(3倍灭活抗原) 二免:灭活苗2ml(3倍灭活抗原) First immunity:2ml inactivated vaccines(3 times inactivated antigen) Second immunity:2ml inactivated vaccines(3 times inactivated antigen) 首免:5周龄仔猪 二免:首免后3周First immunity:5 weeks piglets Second immunity: third week after first immunity 佑本疫苗 至纯至美 2018/12/4 ZFX版权

12 试验分组 Grouping 免疫Immunization 攻毒Challenge 序号 NO. 名称Name 数量 Quantity 免疫剂量(每头) IMD(per head) 免疫日龄 Immune day age 攻毒时间 time 攻毒剂量 dose

3 活+3倍灭活抗原 Live vaccine +3 times inactivated antigen

11 首免:活疫苗1ml(含106.5TCID50) 二免:灭活苗2ml(3倍灭活抗原) First immunity:1ml live vaccine (106.5TCID50) Second immunity:2ml inactivated vaccine (3 times inactivated antigen ) 首免:5周龄仔猪 First immunity: 5-week-old piglet 二免:首免后3周Second immunity:

3 weeks after first immunity 二免后28 天28 days after second immunity Same as above

4 活+佑蓝宝组 Live vaccine+You LanBao

11 首免:活疫苗1ml(含106.5TCID50) 二免:灭活苗2ml(佑蓝宝) First immunity:1ml live vaccine (at least 106.5TCID50) Second immunity:2ml inactivated vaccine(You LanBao)

5 活+3倍佑蓝宝组 Live vaccine+3 times You LanBao

11 首免:活疫苗1ml(含106.5TCID50) 二免:灭活苗2ml(3倍佑蓝宝) First immunity:1ml live vaccine (at least 106.5TCID50) Second immunity:2ml inactivated vaccine(3 times You LanBao)

6 对照组 Control group

12 首免:生理盐水2mlFirst immunity:2ml normal saline 二免:生理盐水2mlSecond immunity:2ml normal saline 1.1 试验设计Test design 佑本疫苗 至纯至美 2018/12/4 ZFX版权

13 分组Grouping 发病数 Morbidity number 死亡数 Death number 保护数 Protection number 试验1组Group