PDF《人类白细胞抗原 -G 在急性白血病患者的表达及意义》

收稿日期院2010-08-15 基金项目院 国家自然科学基金 渊30971300冤曰 广东省社会发展基金 渊2009A030200007冤曰广州市重大科技专项计划渊2008A1-E4011-1冤Supported by National Natural Science Foundation of China (30971300) 作者简介院李 蕊渊1983-冤袁女袁硕士曰曹星辉渊1982-冤袁女袁硕士遥 李 蕊和 曹星辉为共同第一作者 通讯作者院刘启发袁男袁博士生导师袁电话院020-61641612 人类白细胞抗原 -G 在急性白血病患者的表达及意义 李 蕊袁曹星辉袁 刘 灿袁吴秀丽袁凌奕文袁张 钰袁冯 茹袁刘启发渊南方医科大学南方医院血液科袁广东 广州 510515冤 摘要院目的 检测急性白血病患者膜结合型人类白细胞抗原 -G渊mHLA-G冤和分泌型 HLA-G渊sHLA-G冤的表达袁探讨 HLA-G 表达水平的变化对急性白血病发生和发展的影响遥 方法 采用酶联免疫吸附法和流式细胞术分别检测

40 例初 发尧10 例难治复发尧30 例化疗前后患者及

10 例正常人 sHLA-G 和mHLA-G 的表达情况遥 结果 正常人血清中 sHLA-G 的平均水平为 5.

87依2.07 ng/ml袁初发患者为 10.05依6.5 ng/ml尧难治复发患者为 12.32依5.85 ng/ml曰正常人 mHLA-G 的平 均水平为渊0.29依0.20冤 %袁初发患者渊0.60依0.44冤 %尧难治复发患者渊0.77依0.41冤 %遥 白血病初发患者 sHLA-G 和mHLA-G 水平均显著高于正常人组渊P0.05冤遥

15 例1疗程化疗后获得完全缓 解患者 sHLA-G 和mHLA-G 水平显著低于化疗前渊P0.05冤遥 结论 HLA-G 表达水平变化可能与急性白血病的病情发展尧疗效以及预后有关遥 关键词院白血病袁急性曰人类白细胞抗原 -G曰预后 中图分类号院R392;

R733.7 文献标志码院A 文章编号院1673-4254渊2010冤11-2446-03 Clinical implications of HLA-G protein expression in acute leukemia LI Rui, CAO Xing-hui, LIU Can, WU Xiu-li, LING Yi-wen, ZHANG Yu, FENG Ru, LIU Qi-fa Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To detect the expression of membrane-bound HLA-G (mHLA-G) and serum HLA-G (sHLA-G) in acute leukemia patients and investigate the correlation between HLA-G expression and the occurrence and development of acute leukemia. Methods Enzyme-linked immunosorbent assay and flow cytometry were used to detect the expression levels of sHLA-G and mHLA-G in

40 newly diagnosed leukemia cases,

10 refractory and relapsed leukemia cases, and

30 leukemia cases receiving chemotherapy. Ten normal individuals served as the normal control group. Results The mean serum level of sHLA-G in normal individuals was 5.87依2.07 ng/ml, as compared to 10.05 依6.58 ng/ml in newly diagnosed leukemia patients and 12.32依5.85 ng/ml in refractory and relapsed cases. The mean level of mHLA-G in normal individuals, newly diagnosed cases, and refractory and relapsed cases were (0.29依0.20)%, (0.60依0.44)%, and (0.77依0.41)%, respectively. The mean levels of sHLA-G and mHLA-G were significantly higher in the newly diagnosed cases than that in the normal controls (P