PDF《patients》

4 and 5q, 7q36, 11q, 12p, 16, 17q and

19 and gain of 1p in four of

29 cells. FISH analysis showed deletion of 5q in

27 of

200 interphase cells. Molecular analysis performed directly on core biopsy tissue containing ~ 60% blasts showed 480% donor DNA (Figure 1). Case

3 A 58-year-old man with primary refractory follicular lymphoma received a matched sibling donor transplant, and developed anemia (Hemoglobin 13.4 g/dL) and thrombocytopenia (59 000/ μL)

6 years later. His marrow showed myelodysplastic syndrome (MDS) with ringed sideroblasts. Cytogenetics later revealed monosomy

7 in six cells and deletion

20 in nine cells (non- overlapping). Chimerism studies from an adequate aspirate sample showed 99% donor DNA and 1% recipient DNA, consistent with DDN. Case

4 An 81-year-old woman with a history of diffuse large B-cell lymphoma (DLBCL) underwent CHOP and achieved CR, but

3 years later, developed chronic myelomonocytic leukemia (CMML) with isochromosome 17q. She received topotecan and Ara-C followed by allo-HCT from a male-matched unrelated donor. Chimerism assessment was consistent with complete donor origin (46,XY). Eight years post transplant, she developed '

therapy-related'

AML. The marrow biopsy showed no signi?cant ?brosis, and aspirate demonstrated 77% blasts. Although chimerism studies were not available after development of AML, karyotype of her marrow continued to show only XY chromosomes, consistent with DDN. The patient died

2 months after the AML diagnosis. Case

5 An 82-year-old woman with history of B-cell prolymphocytic leukemia (B-PLL) was treated with ?udar........