编辑: kr9梯 | 2014-03-31 |
筛选;
纤 维素酶;
鉴定 本文作者相关文章 PubMed 一株嗜热纤维素分解菌的分离及其酶学性质的初探 曲小爽1,2,顿宝庆1,郭明鸣1,2,宋立立1,3,许修宏2, 张保明1,路明1,李桂英1 (1.
中国农业科学院作物科学研究所,国家农作物基因资源与基因改良国家重大科学工程,中国农业科学 院生物质能源研究中心, 北京 100081|2.东北农业大学生命科学学院, 哈尔滨 150030;
3.西北农林科技大学, 陕西 杨凌 712100) 摘要: 利用海南热泉附近腐烂朽木及土壤,筛选分离到一株具有较高纤维素酶活性的纤维素分解菌WQ-50,经生理生化特性 及遗传分析初步鉴定其为环状芽孢杆菌属菌株.当以复筛培养基发酵培养时, 该菌株在55℃、pH 6条件下生长较 好.在该条件下,以经碱预处理的玉米秸秆粉为唯一碳源发酵培养5 d时产酶达到最大值,羧甲基纤维素酶活性为6.8 IU/mL,滤纸酶活性为3.5 IU/mL. 关键词: 嗜热纤维素分解菌;
筛选;
纤维素酶;
鉴定 Initial Studies on Isolation of a Thermophilic Cellulose-degrading Bacteria and its Enzymology Characteristics QU Xiao-shuang1,2, DUN Bao-qing1, GUO Ming-ming1,2, SONG Li-li1,3, XU Xiu-hong2, Zhang Bao-ming1, LU Ming1, Li Gui-ying1 (1.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Biomass Energy Research Center, Chinese Academy of Agricultural Sciences, Beijing 100081;
|2.College of Life Sciences, Northeast Agricultural University, Harbin 150030|3. Northwest |A &|F University, Shannxi Yangling 712100, China) Abstract: WQ-50 was isolated from decayed wood and soil near hot spring in Hainan. It was identified as Bacilluscirculans by preliminary physiological and biochemical characteristics and genetic analysis. When fermented by CMC-Na culture medium, strain WQ-50 strain could grow well under PH
6 and 55℃. Under this condition, when fermented by zymolytic culture medium with alkalized pretreated corn stalk powder as the sole carbon source, enzyme production reached maximum after
5 days, the CMCase activity of WQ-50 is 6.8 IU/mL and FPA activiy of WQ-50 is 3.5 IU/mL. Keywords: thermophilic cellulose-degrading bacteria screen cellulose enzyme identification 收稿日期 2008-10-30 修回日期 2008-11-12 网络版发布日期 DOI: 基金项目: "十一五"国家科技支撑计划项目(2006BAD07A04);
农业部农村能源综合建设项目(2130138-0-08);
中央级 公益性科研院所基本科研业务费专项资金(2060302-13)资助. 通讯作者: 李桂英,研究员,从事生物质能源研究.E-mail:[email protected].顿宝庆,助理研究员,从事能源和 环境微生物研究.E-mail:[email protected] 作者简介: 曲小爽,硕士研究生,研究方向为微生物学.E-mail:[email protected]. 作者Email: 参考文献: 本刊中的类似文章 文章评论 反馈人邮箱地址 反馈标题验证码 Copyright by 中国农业科技导报