PDF《Hepatitis, not for Septic Shock》

200 ?l). Experimental Protocol Mice were injected intraperitoneally with either LPS alone (40 mg/kg), LPS (10 ?g/kg or

50 ?g/kg) in combination with D-GalN (800 mg/kg), or murine recombinant TNF-? (20 ?g/kg) with D-GalN (800 mg/kg) in a solution of

200 ?l per dose. YVAD-treated mice received YVAD-CMK in

200 ?l sterile PBS either

2 h before, simultaneously, or

2 h after LPS or TNF-? administration. Control groups in the LPS, LPS/D-GalN, and TNF-? received

200 ?l sterile saline intraperito- neally. Mice were exsanguinated by retro-orbital puncture

90 min and

4 h after LPS cha........