PDF《河北省和山东省黄瓜霜霉病菌对氟吡菌胺的抗性》

植物保护学报 Journal of Plant Protection, 2019,

46 (2) : 385-392 DOI: 10.

13802/j.cnki.zwbhxb.2019.2017220 河北省和山东省黄瓜霜霉病菌对氟吡菌胺的抗性 及常规药剂对黄瓜霜霉病的田间防效 王文桥1* 韩秀英1 吴杰1 赵建江1 孟润杰2 路粉1 (1. 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有 害生物综合治理重点实验室, 保定 071000;

2. 保定职业技术学院, 河北 保定 071051) 摘要: 为明确黄瓜霜霉病菌Pseudoperonospora cubensis对氟吡菌胺的抗性时空动态, 2011―2016年 从河北省和山东省黄瓜主产区采集1 821株霜霉病菌, 采用叶盘漂浮法检测供试菌株对氟吡菌胺的 敏感性, 并以茎叶喷雾法评估5种常规药剂对黄瓜霜霉病的田间防效.结果表明, 河北省和山东省 黄瓜主产区的黄瓜霜霉病菌对氟吡菌胺已普遍产生了低度抗性, 平均抗性倍数为5.86;

所有供试菌 株的抗性频率为 51.89%, 其中低抗菌株及中抗菌株分别占 36.18% 和15.71%;

平均抗性指数为 0.34, 每年检测的抗性频率、 抗性倍数及抗性指数呈增长趋势, 且随着监测区域的变化而变化.在 河北省定兴县和山东省寿光市进行的田间防效试验结果显示, 按推荐剂量喷施, 687.5 g/L氟吡菌 胺・霜霉威盐酸盐SC对黄瓜霜霉病防效显著高于常规对照药剂68%精甲霜灵 ・ 代森锰锌WG、 58% 甲霜灵 ・ 代森锰锌WP、 80%代森锰锌WP及250 g/L嘧菌酯SC的防效, 但防效明显下降, 由2011年的92.58%~93.31% 降至2016年的80.07%~80.82%.表明需要制定和实施抗药性治理对策, 如限制 山东和河北2省黄瓜的每个生长季节中687.5 g/L氟菌 ・ 霜霉威SC的使用不超过2次, 而且要与不 同作用机理的卵菌杀菌剂交替使用防治黄瓜霜霉病. 关键词: 黄瓜霜霉病菌;

氟吡菌胺;

抗性;

时空动态;

田间防效 Resistance of Pseudoperonospora cubensis to fluopicolide and control efficacy of conventional fungicides against cucumber downy mildew in Hebei and Shandong provinces, China Wang Wenqiao1* Han Xiuying1 Wu Jie1 Zhao Jianjiang1 Meng Runjie2 Lu Fen1 (1. Key Laboratory for Integrated Management of Crop Pests in Northern Regions of North China, Ministry of Agriculture and Rural Affairs, Integrated Pest Managment Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;

2. Baoding College of Technology, Baoding 071051, Hebei Province, China) Abstract: To clarify the temporal and spatial dynamics of the resistance Pseudoperonospora cubensis to fluopicolide in Hebei and Shandong provinces of China, the sensitivities to fluopicolide in

1 821 P. cubensis strains collected from main cucumber production areas of Hebei and Shandong provinces from

2011 to

2016 were measured by leaf disc floating tests, and the control efficacies of five conventional fungicides against cucumber downy mildew were assayed by spraying stems and leaves in the fields. The results showed that P. cubensis widely developed low resistance to fluopicolide all over the main cucumber production areas of Hebei and Shandong, with an average resistance factor of 5.86. The 基金项目: 国家重点研发计划 (2016YFD0201006) , 国家公益性行业 (农业) 科研专项 (201003004) , 农业部农作物病虫鼠害疫情监测与防治项目 (10162130108235057) * 通信作者(Author for correspondence) , E-mail: [email protected] 收稿日期: 2017-11-18