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黑龙江省农业科学院作物育种研究所, 黑龙江 哈尔滨 150086;
2. 黑龙江省农业科学院生物 技术研究所, 黑龙江 哈尔滨 150086;
3. 黑龙江省农业科学院玉米研究中心, 黑龙江 哈尔滨
150086 摘要: 以小麦转HMW-GS1Dx5+1Dy10基因获得的1Ax1和1Ax2 * 近等基因系08K860(HWM-GS组成为 1,7+9,5+10)和08K871(HWM-GS亚基组成为2 * ,7+9,5+10)为材料,研究了近等基因系籽粒品质、粉质仪参数、 拉伸仪参数和色度仪参数等方面的差异.研究结果表明,近等基因系08K860和08K871的上述性状差异很小,经统计 分析差异未达显著水平.近等基因系08K860和08K871遗传背景相近,1Ax1和1Ax2 * 亚基对品质的贡献率相同.在 小麦品质育种上应同样重视具有1Ax1亚基后代的选择. 关键词: 小麦 高分子量麦谷蛋白亚基 近等基因系 等位变异 品质 CHARACTERISTIC DIFFERENCES OF WHEAT 1Ax1 AND 1Ax2* NILS SUN Yan
1 , ZHANG Hong-ji
1 , WANG Guang-jin
2 , LIU Dong-jun
1 , QI Qian-qian
1 , YANG Shu-ping
1 , ZHAO Wei
3 , GUO Yi-fan
1 , MA Shu-mei
1 , LIU Wen-lin
1 1. Crop Breeding Istitute of Heilongjiang of Agricultrual Science, Harbin, Heilongjiang 150086;
2. Biotechnique Istitute of Heilongjiang of Agricultural Science, Harbin, Heilongjiang 150086;
3. Maize Research Center for Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang
150086 Abstract: Wheat 1Ax1 and 1Ax2 * near-isogenic lines (NILs) 08K860(HMW-GS 1,7+9,5+10) and 08K871 (HMW-GS
2 * ,7+9,5+10) were obtained by HMW-GS 1Dx5+1Dy10 gene transformation, the quality characteristics between them were compared in this study. The results showed that the differences in seed quality parameters, farinogram parameters, extensigram parameters and Minolta CR-310 parameters between 1Ax1 and 1Ax2 * were not significant, it is considered that 08K860 and 08K871 are similar in hereditary background and have similar contribution to quality. Therefore, they should have equal importance to wheat breeding for quality improvement. Keywords: wheat HMW-GS near-isogenic lines(NILS) isogenetic mutation quality 收稿日期 2011-09-08 修回日期 2011-12-22 网络版发布日期 DOI: 基金项目: 黑龙江省自然科学基金(C2007-09);
黑龙江省基金重点(ZJN0704);
农业部转基因生物新品种培育科技重大专项 (2008ZX08002-004) 通讯作者: 张宏纪(1969-),男,黑龙江抚远人,博士,研究员,主要从事小麦诱变与生物技术育种.Tel:0451- 86668741;