PDF《β-Tubulin (1E1) Mouse mAb》

正能生物 β-Tubulin (1E1) Mouse mAb 货号:200608 Antibody type Loading control antibody Conjugation Unconjugated Modification Unmodification Isotype IgG1 Host Mouse Application WB , ICC/IF Purification Affinity purified Cross reactivity Human , Mouse , Monkey , Goat , Hamster , Rat , Chlamydomonas Reinhardtii Gene name tubb Alternative names M40;

TUBB1;

TUBB5;

OK/SW-cl.

56 Gene symbol beta

4 tubulin;

Beta

5 tubulin;

beta Ib tubulin;

M40;

TUBB1;

TUBB5;

tubulin beta

1 chain;

Tubulin beta

4 chain;

tubulin beta

5 chain;

Tubulin beta;

Tubulin beta chain;

tubulin beta polypeptide. Description Swiss-Prot Acc.P07437.Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha- chain. Immunogen Purified recombinant human Tubulin beta protein fragments expressed in E.coli. Clonality Monclonal antibody Target This antibody detects endogenous levels of Tubulin beta and does not cross-react with related proteins. Gene ID

203068 Sequence similarity Belongs to the tubulin family. Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol. Clone number 1E1-E8-H4 Subcellular location Cytoplasm, Cytoskeleton, Microtubule Storage Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. Dilution WB 1:5000,ICC 1:500,IF 1:100 SwissProt ID P07437 Molecular Weight 55kDa Source Human Application image Western blot detection of Tubulin beta in COS7,LNCap,MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa. Immunocytochemistry staining of HeLa cells fixed with 4% paraformaldehyde and using anti-Tubulin beta mouse mAb (dilution 1:500). Tubulin beta antibody's stability test under 37℃

50 hours compare to 4℃

50 hours. Western blot detection of Tubulin beta in goat intestine,goat skeletal muscle,goat spleen and goat lung cell lysates using Tubulin beta mouse mAb (1:5000-1:10000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa. Western blot detection of Tubulin beta in Goat skeletal muscle,CHO-k1,COS7,C6,3T3 and K562 cell lysates using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size:55KDa.Observed band size:55KDa.Exposure time:10sec. Western blot detection of beta Tubulin in Chlamydomonas cell lysates using beta Tubulin mouse mAb (1:1000 diluted).Predicted band size:55KDa.Observed band size:55KDa. 公司地址:成都市高新区科园南路9号附1号爱斯特大厦一层 联系

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