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电话: 400-6721-600 Human Relaxin-2 ELISA Kit This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK11601 C

48 T EK11602 C

96 T EK11603 C

2 *96 T EK11604 C

5 *96 T EK11605 C

10 *96 T For the quantitative determination of human Relaxin-2 concentration in cell culture supernates, serum and plasma. For research use only. Not for use in diagnostic procedures. Human Relaxin-2 ELISA Kit 08/2016

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电话: 400-6721-600 Human Relaxin-2 ELISA Kit Table of Contents Introduction Description

1 Principle of the Assay

1 Limitations of the Procedure

1 General Information Materials Provided

3 Other Supplies required

3 Storage

5 Precaution

7 Technical Hints

9 Assay Protocol Sample Collection and Storage

9 Reagent Preparation 11,13 Assay Procedure

15 Analysis Calculation of Results

17 Typical Data

17 Sensitivity

19 Precision

19 Recovery

21 Linearity

21 Calibration

21 Sample Values

23 Specificity

23 Assay Procedure Summary

25 目录 产品介绍 背景介绍

2 检测原理

2 试剂盒检测的局限

2 基本信息 试剂盒提供的材料

4 未提供的材料设备

4 贮存

6 注意事项

8 技术要点

10 检测步骤 样本的收集和贮存

10 试剂准备 12,

14 检测步骤

16 分析 结果计算

18 典型数据

18 灵敏度

20 精密度

20 回收率

22 稀释线性

22 校准

22 样本值

24 特异性

24 检测步骤概要

26

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电话: 400-6721-600 Human Relaxin-2 ELISA Kit DESCRIPTION Human Relaxin-2 is synthesized and secreted as a preprohormone that is

18 kDa in size and

185 amino acids in length. It is a peptide hormone best known for its important physiological roles during pregnancy. The role of relaxin in pregnancy physiology differs considerably between mammals. In humans and other primates, relaxin does play a role in pregnancy, albeit an earlier role of modulating endometrial physiology during implantation. In rodents there is a major pre-labor surge in circulating relaxin levels, corresponding to its role in parturition;

however, in humans and other primates, the serum levels of relaxin are highest during the first trimester. In women, the ovarian corpus luteum is the source of circulating Relaxin-2. It should be noted that Relaxin-2 is also present in males and is synthesized by non-reproductive organs such as the kidney, heart, and lungs suggesting a more global contribution for this hormone. Relaxin-2 initiates its pleiotropic effects through multiple pathways on a variety of cell types. It confers activity by binding to the leucine-rich G protein-coupled receptor LRG7, and with significantly less affinity to LRG8 PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Relaxin-2 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for Relaxin-2 are pipetted into the wells and Relaxin-2 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Relaxin-2 bound in the initial step. The color development is stopped and the intensity of the color is measured. LIMITATIONS OF THE KIT FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. The kit should not be used beyond the expiration date on the kit label. Do not mix or substitute reagents with those from other lots or sources. If samples generate values higher than the highest standard, dilute the samples with Assay Buffer and repeat the assay. If cell culture supernate samples require large dilution, perform an intermediate dilution in culture medium. Any variation in standard dilution, operator, pipetting technique, washing techniques, incubation time or temperature, and kit age can cause variation in binding. This assay is designed to eliminate interference by factors present in biological samples. Until all factors have been tested in the SunnyELISA immunoassay, the possibility of interference cannot be excluded. 背景介绍 人松弛素