PDF《The Russian ASF experience:》

The Russian ASF experience: epidemiology, prevention and strategies for disease control 09th of April 2019, Beijing, PRC Denis Kolbasov, Russia 俄罗斯非洲猪瘟的主要病原特点、 防控策略及防控效果分析 ASF distribution overview ASF病毒分布概览 4/26/2019 WWW.

VNIIVVIM.RU

2 Beginning 4/26/2019 WWW.VNIIVVIM.RU

3 俄罗斯ASF病毒爆发点图示(2008至2019年) 家猪 野猪 2008-2018 ASF ASF in Russia March

2019 俄罗斯地区ASF病毒情况(2019年3月) 4/26/2019 WWW.VNIIVVIM.RU

4 家猪 野猪 受感染区域 4/26/2019 WWW.VNIIVVIM.RU

5 2019年图示 家猪 野猪 猪肉生产 基因组 受感染国家 üInfected animal or carcass 受感染的动物或动物尸体 üBackyard 散养猪 üSettlement 解决方案 4/26/2019 WWW.VNIIVVIM.RU

6 No clear definition of case/outbreak 病毒爆发后没有明确定义的方面 STABILITY OF VIRUS 病毒的稳定性 Clinical forms of ASF Acute 强毒 Mild 弱毒 4/26/2019 WWW.VNIIVVIM.RU

8 ASF病毒的临床表现 体温 白细胞总数 ASFV Strains

2017 ? ASFV_VLADIMIR_2017_WB ? ASFV_KUBAN_2017_DP ? ASFV_SARATOV_2017_DP ? ASFV_IRKUTSK_2017_DP ? ASFV_OMSK_2017_DP 4/26/2019 WWW.VNIIVVIM.RU

9 ASFV 2017系列病毒毒株 Bioassay ?

3 pigs in every group were infected with 10*3 HAU IM. 每个群体有3头猪只感染了10*3 HAU IM 型ASF病毒;

? Clinical symptoms and temperature were registered daily. 每天登记猪只的临床症状及体温情况. 4/26/2019 WWW.VNIIVVIM.RU

10 猪只遇到的挑战 Temperature profiles ASFV_Saratov_2017_DP ASFV_Kuban_2017_DP ? Mean time to fever平均发热时间(TTF) C 3.5 dpi ? Mean time of fever平均发热时间 C 5.7 days 4/26/2019 WWW.VNIIVVIM.RU

11 体温资料 Clinical score 临床评分 ASFV_Saratov_2017_DP ASFV_Kuban_2017_DP ? Mean time to death 平均死亡时间 (TTD) C 8.5 days 4/26/2019 WWW.VNIIVVIM.RU

12 MOLECULAR CHARACTERIZATION OF ASFV STRAINS

2017 ? ASFV_VLADIMIR_2017_WB ? ASFV_IRKUTSK_2017_DP ? ASFV_SARATOV_2017_DP ? ASFV_KUBAN_2017_DP ? ASFV_OMSK_2017_DP ASFV IGR II>ASFV IGR I 4/26/2019 WWW.VNIIVVIM.RU

13 ASFV 2017病毒毒株的分子特性 Align fragments of IGR I73R/I329L OF ASFV STRAINS 2016-2017 ASFV 2016-2017病毒毒株IGRI73R/I329L的基因片断对比 4/26/2019 WWW.VNIIVVIM.RU

14 üSome additional SNP's were identified in different ASFV genes regardless ASFV strains origin 不管ASF病毒的毒株来源,一些附加的SNP被证明处在不同的ASF病毒基因组;

üDespite the fact, that the number of nucleotide mismatches in currently circulating ASFV strains is dramatically increasing there is no clear evidence of virus attenuated phenotype in Russia 尽管事实如此, 但核苷酸的数量与现存的ASF病毒毒株无法匹配,并呈显著增 长的趋势.没有明显的证据表明俄罗斯区域的病毒呈减毒表型态. 4/26/2019 WWW.VNIIVVIM.RU

15 ASF in wild boars 感染ASF病毒的野猪 4/26/2019 WWW.VNIIVVIM.RU

16 Wild boar spring census 2008-2017 (by Federal Districts) 2008-2017年俄罗斯联邦野猪春季普查情况 4/26/2019 WWW.VNIIVVIM.RU

17 ASF and wild boar population ASF及野猪猪群 4/26/2019 WWW.VNIIVVIM.RU

18 Regions where ASF was registered in wild boar earlier and currently is not notified (for at least

2 years) 俄联邦各区域早期即登记野猪感染ASF病毒情况,但最 近两年未有报导. Comparison the number of ASF cases in WB in

3 years period 4/26/2019 WWW.VNIIVVIM.RU

19 三年期间感染ASF病毒的野猪数量对比图 Correlation between WB density and number of ASF cases 4/26/2019 WWW.VNIIVVIM.RU