PDF《SunnyELISA》

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电话: 400-6721-600 Mouse CCL20/MIP-3α ELISA Kit SunnyELISA This package insert must be read entirely before using this product. Catalog Number EK22111 C

48 T EK22112 C

96 T EK22113 C

2 *96 T EK22114 C

5 *96 T EK22115 C

10 *96 T For the quantitative determination of mouse Chemokine (C-C motif) Ligand

20 (CCL20/MIP-3α) concentration in cell culture supernates, serum and plasma. For research use only. Not for use in diagnostic procedures. Mouse CCL20/MIP-3α ELISA Kit 01/2016 For proper performance, use the insert provided with each individual kit received.

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电话: 400-6721-600 Mouse CCL20/MIP-3α ELISA Kit Table of Contents Introduction Description

1 Principle of the Assay

1 Limitations of the Procedure

1 General Information Materials Provided

3 Other Supplies required

3 Storage

5 Precaution

7 Technical Hints

9 Assay Protocol Sample Collection and Storage

9 Reagent Preparation 11,13 Assay Procedure

15 Analysis Calculation of Results

17 Typical Data

17 Sensitivity

19 Precision

19 Recovery

21 Linearity

21 Calibration

21 Sample Values

23 Specificity

23 Assay Procedure Summary

25 目录 产品介绍 背景介绍

2 检测原理

2 试剂盒检测的局限

2 基本信息 试剂盒提供的材料

4 未提供的材料设备

4 贮存

6 注意事项

8 技术要点

10 检测步骤 样本的收集和贮存

10 试剂准备 12,

14 检测步骤

16 分析 结果计算

18 典型数据

18 灵敏度

20 精密度

20 回收率

22 稀释线性

22 校准

22 样本值

24 特异性

24 检测步骤概要

26

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电话: 400-6721-600 Mouse CCL20/MIP-3α ELISA Kit DESCREPTION Chemokine (C-C motif) ligand

20 (CCL20), also known as macrophage inflammatory protein-3α (MIP-3α), is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. Cells known to express CCL20 include monocytes, macrophages, microglia and astrocytes, mast cells, keratinocytes, intestinal epithelium, osteoblasts, fibroblasts, endothelial cells, and bronchial epithelial cells. CCL20 is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. CCL20 elicits its effects on its target cells by binding and activating the chemokine receptor CCR6. CCL20 may play a critical role in autoimmune pathogenesis of the central nervous system. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse CCL20 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for CCL20 are pipetted into the wells and CCL20 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of CCL20 bound in the initial step. The color development is stopped and the intensity of the color is measured. LIMITATIONS OF THE KIT FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. The kit should not be used beyond the expiration date on the kit label. Do not mix or substitute reagents with those from other lots or sources. If samples generate values higher than the highest standard, dilute the samples with Assay Buffer and repeat the assay. If cell culture supernate samples require large dilution, perform an intermediate dilution in culture medium. Any variation in standard dilution, operator, pipetting technique, washing techniques, incubation time or temperature, and kit age can cause variation in binding. This assay is designed to eliminate interference by factors present in biological samples. Until all factors have been tested in the SunnyELISA immunoassay, the possibility of interference cannot be excluded. 背景介绍 趋化因子配体