PDF《#3087》

#3087 Store at -20°C C/EBPb (LAP) Antibody rev.

01/05/16 W Endogenous H, M, (R) 35-38 kDa mouse LAP, 45-49 kDa human LAP Rabbit** Background: CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular dif- ferentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/ EBPa, -b, -d, -g, -e and -z) and are distributed in a variety of tissues (1). There are two forms of C/EBPb, the

38 kDa liver activating protein (LAP) and the

20 kDa liver inhibitory protein (LIP) which may be products of alternative transla- tion. The

38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPb transcriptional activity (2). Phosphorylation of C/EBPb at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at Ser105 of rat C/EBPb, a unique site only present in the rat sequence, seems essential for rat C/EBPb activation (6). Specificity/Sensitivity: C/EBPb (LAP) Antibody detects endogenous levels of total C/EBPb, the p38 and p36 LAPs, but not the p20 LIP. This antibody does not cross-react with C/EBPa, -d, -g, -e or -z. Source/Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide correspond- ing to the amino-terminal sequence of human C/EBPb. Antibodies are purified by protein A and peptide affinity chromatography Background References: (1) Lekstrom-Himes, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545C28548. (2) Calkhoven, C.F. et al. (2000) Genes Dev. 14, 1920C1932. (3) Wegner, M. et al. (1992) Science 256, 370C373. (4) Trautwein, C. et al. (1993) Nature 364, 544C547. (5) Nakajima, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 2207C2211. (6) Buck, M. et al. (1999) Mol. Cell 4, 1087C1092. Storage: Supplied in

10 mM sodium HEPES (pH 7.5),

150 mM NaCl,

100 ?g/ml BSA and 50% glycerol. Store at C20°C. Do not aliquot the antibody. *Species cross-reactivity is determined by western blot. ** Anti-rabbit secondary antibodies must be used to detect this antibody. Recommended Antibody Dilutions: Western Blotting 1:1000 For application specific protocols please see the web page for this product at www.cellsignal.com. Please visit www.cellsignal.com for a complete listing of recommended companion products. Orders n 877-616-CELL (2355) [email protected] Support n 877-678-TECH (8324) [email protected] Web n www.cellsignal.com Applications Species Cross-Reactivity* Molecular Wt. Source ?

2010 Cell Signaling Technology, Inc. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Entrez-Gene ID #1051 Swiss-Prot Acc. #P17676 C + + C/EBPb Transfection Human Mouse kDa

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30 Human C/EBPb (LAP) Mouse C/EBPb (LAP)

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48 Induction (hr) kDa

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20 10 C/EBPβ (LAP) Western blot analysis of extracts from 3T3-L1, differentiated for the indicated times, using C/EBPb (LAP) Antibody. Western blot analysis of extracts from COS cells, untransfected or transfected with human or mouse C/EBPb (LAP), using C/EBPb (LAP) Antibody. For Research Use Only. Not For Use In Diagnostic Procedures. Species Cross-Reactivity Key: H―human M―mouse R―rat Hm―hamster Mk―monkey Mi―mink C―chicken Dm―D. melanogaster X―Xenopus Z―zebrafish B―bovine Dg―dog Pg―pig Sc―S. cerevisiae Ce―C. elegans Hr―horse All―all species expected Species enclosed in parentheses are predicted to react based on 100% homology. Applications Key: W―Western IP―Immunoprecipitation IHC―Immunohistochemistry ChIP―Chromatin Immunoprecipitation IF―Immunofluorescence F―Flow cytometry E-P―ELISA-Peptide