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电话: 400-6721-600 Human BDNF ELISA Kit This package insert must be read entirely before using this product. For proper performance, use the insert provided with each individual kit received. Catalog Number EK11271 C

48 T EK11272 C

96 T EK11273 C

2 *96 T EK11274 C

5 *96 T EK11275 C

10 *96 T For the quantitative determination of human Brain-Derived Neurotrophic Factor (BDNF) concentration in cell culture supernates, serum and plasma. For research use only. Not for use in diagnostic procedures. Human BDNF ELISA Kit 06/2016

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电话: 400-6721-600 Human BDNF ELISA Kit Table of Contents Introduction Description

1 Principle of the Assay

1 Limitations of the Procedure

1 General Information Materials Provided

3 Other Supplies required

3 Storage

5 Precaution

7 Technical Hints

9 Assay Protocol Sample Collection and Storage

9 Sample Preparation

11 Reagent Preparation 11,13 Assay Procedure

15 Analysis Calculation of Results

17 Typical Data

17 Sensitivity

19 Precision

19 Recovery

21 Linearity

21 Calibration

21 Sample Values

23 Specificity

23 Assay Procedure Summary

25 目录 产品介绍 背景介绍

2 检测原理

2 试剂盒检测的局限

2 基本信息 试剂盒提供的材料

4 未提供的材料设备

4 贮存

6 注意事项

8 技术要点

10 检测步骤 样本的收集和贮存

10 样本准备

12 试剂准备 12,

14 检测步骤

16 分析 结果计算

18 典型数据

18 灵敏度

20 精密度

20 回收率

22 稀释线性

22 校准

22 样本值

24 特异性

24 检测步骤概要

26

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电话: 400-6721-600 Human BDNF ELISA Kit DESCREPTION Brain-derived neurotrophic factor (BDNF), is a member of the neurotrophin family of growth factors, which are related to the Nerve Growth Factor. Neurotrophic factors are found in the brain and the periphery. BDNF is made in the endoplasmic reticulum and secreted from dense-core vesicles. In the brain, it is active in the hippocampus, cortex, and basal forebrain. It is also expressed in the retina, motor neurons, the kidneys, saliva, and the prostate. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. BDNF alterations may play a role in the pathogenesis of schizophrenia. It is also related with depression, eczema, epilepsy, alzheimer'

s disease, obesity, aging, congenital central hypoventilation syndrome and post-chemotherapy cognitive impairment. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human BDNF has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for BDNF are pipetted into the wells and BDNF present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured. LIMITATIONS OF THE KIT FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. The kit should not be used beyond the expiration date on the kit label. Do not mix or substitute reagents with those from other lots or sources. If samples generate values higher than the highest standard, dilute the samples with Assay Buffer and repeat the assay. If cell culture supernate samples require large dilution, perform an intermediate dilution in culture medium. Any variation in standard dilution, operator, pipetting technique, washing techniques, incubation time or temperature, and kit age can cause variation in binding. This assay is designed to eliminate interference by factors present in biological samples. Until all factors have been tested in the ELISA immunoassay, the possibility of interference cannot be excluded. 背景介绍 脑源性神经营养因子(BDNF)是生长因子神经营养素家族中的一员,与神经生长因子相关.神 经营养因子存在于脑及外周.BDNF 在内质网中加工合成,由致密核心囊泡分泌.它在大脑中的 海马、皮层和基底前脑区域活跃,同样也在视网膜、运动神经元、肾脏、唾液和前列腺中表达. BDNF 作用于中枢神经系统和外周神经系统的某些神经元,帮助现存神经元的存活、促进新 生神经元和突触的生长和分化. BDNF 的改变可能在精神分裂症的发病中起作用, 与抑郁、 湿疹、 癫痫、阿尔茨海默病、肥胖、衰老、先天性中枢性低通气综合征和化疗后认知功能障碍有关. 检测原理 本试剂盒采用双抗体夹心酶联免疫吸附检测技术.特异性抗人 BDNF 单克隆抗体预包被在高 亲和力的酶标板上.酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本 中存在的 BDNF 与固相抗体和检测抗体结合.洗涤去除未结合的物质后,加入辣根过氧化物酶标 记的链霉亲和素(streptavidin-HRP).洗涤后,加入显色底物 TMB,避光显色.颜色反应的深浅与 样本中 BDNF 的浓度成正比.加入终止液终止反应,在450 nm 波长(参考波长