PDF《Product datasheet》

产 产品名称 品名称 重组人htrA4蛋白 蛋白 蛋白长 长度 度Protein fragment 性 性质 质Recombinant 来源 来源 Escherichia coli Accession P83105 种属 种属 Human 序列 序列 MRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEK VAPSVVHVQL WGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWI EVVLQNGARY EAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEF VVALGSPFSL QNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNS GGPLVNLDGD VIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKGKAFS NKKYLGLQML SLTVPLSEELKMHYPDFPDVSSGVYVCKVVEGTAAQSSGL RDHDVIVNIN GKPITTTTDVVKALDSDSLSMAVLRGKDNLLLTVIPETIN HHHHHH 分子量 分子量

38 kDa including tags 氨基酸 氨基酸

138 to

476 标签 标签 His tag C-Terminus 氨基酸序列 氨基酸序列 生物活性 生物活性 Proteolytic activity of ab134446 was documented by digestion of ?- casein.

0.5 mg ?-casein/ml Product datasheet Recombinant human htrA4 protein ab134446

2 图像 概述 概述 描述 描述 技 技术 术指 指标 标Our Abpromise guarantee covers the use of ab134446 in the following tested applications. The application notes include recommended starting dilutions;

optimal dilutions/concentrations should be determined by the end user.

1 are completely digested by

10 ?g/ml HtrA4 within

24 hours at 37°C. 应 应用 用SDS-PAGE Functional Studies Inhibition Assay 纯 纯度 度>90% by SDS-PAGE. 形式 形式 Liquid 补 补充 充说 说明 明Upon freezing, a faint precipitate may form which contains htrA4. This precipitate was shown to contain catalytically active htrA4 in a casein digest. Therefore, we recommend vortexing ab134446 before use. 稳 稳定性和存 定性和存储 储Shipped on dry ice. Upon delivery aliquot and store at -80?C. Avoid freeze / thaw cycles. pH: 7.50 Preservative: 0.816% Imidazole Constituents: 0.64% Tris HCl, 15% Glycerol, 0.704% Sodium chloride This product is an active protein and may elicit a biological response in vivo, handle with caution. 功能 功能 Serine protease. 序列相似性 序列相似性 Belongs to the peptidase S1B family. Contains

1 IGFBP N-terminal domain. Contains

1 Kazal-like domain. Contains

1 PDZ (DHR) domain. 细 细胞定位 胞定位 Secreted. 制 制备 备和 和贮 贮存 存常常规 规信息 信息 图 图片 片2SDS-PAGE - Recombinant human htrA4 protein (ab134446) SDS-PAGE analysis of ab134446 (1?g). Due to minor N-terminal truncation of the protein it may appear as a double band. SDS-PAGE - Recombinant human htrA4 protein (ab134446) Hydrolysis of ?-Casein by ab134446. ?-Casein (0.5 mg/ml) was incubated in

50 mM Tris-HCl, pH 7.5,

150 mM NaCl, 0.5 mM CaCl2 with or without htrA4 (10 ?g/ml). After indicated time intervals

15 ?l aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE. Lane 1: BSA-only control,

0 hours Lane 2: BSA-only control,

24 hours Lane 3: 1?g ab134446,

0 hours Lane 4: 1?g ab134446,

6 hours Lane 5: 1?g ab134446,

24 hours Please note: All products are "FOR RESEARCH USEONLY. NOTFOR USEIN DIAGNOSTIC PROCEDURES" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for

12 months from date of delivery Response to your inquiry within

24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit https://www.abcam.cn/abpromise or contact our technical team.