PDF《htrb-1》

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1000 1500 htrb-3 htrb-1 htrb-2 htrb-3 template A B C D Supplementary Figure

1 Supplementary Table

1 A Cytokine fold AP-1 activation fold NFkB activation Interferon- 3.

35 ±6% 1.23 ±13% Interferon- 1.67 ±5% 1.25 ±6% IL-2 1.02 ±17% 1.00 ±34% IL-3 1.91 ±7% 1.22 ±11% IL-4 1.40 ±26% 0.95 ±8% IL-5 8.24 ±8% 1.74 ±2% IL-6 0.96 ±11% 1.05 ±6% IL-7 0.76 ±24% 1.10 ±6% IL-9 1.36 ±16% 0.86 ±2% IL-10 0.78 ±5% 0.89 ±5% IL-11 0.69 ±8% 0.76 ±9% IL-12 2.14 ±11% 0.69 ±13% IL-13 0.84 ±10% 1.08 ±21% IL-15 1.34 ±10% 1.76 ±7% bFGF 0.72 ±14% 1.14 ±9% EGF 0.84 ±4% 1.28 ±6% Ins. like GF 0.69 ±16% 1.07 ±3% TGF- 13.37 ±13% 6.61 ±5% Oncostatin M 0.72 ±18% 0.87 ±6% M-CSF 1.52 ±5% 1.20 ±16% G-CSF 0.96 ±14% 1.06 ±15% GM-CSF 1.04 ±15% 1.38 ±21% PDGF-BB 9.06 ±6% 0.76 ±18% LIF 0.73 ±7% 0.85 ±10% Scatter factor 3.30 ±8% 1.03 ±4% PMA 10.37 ±9% 5.16 ±15% B Cytokine AP-1 inhibition NFB inhibition Scatter factor 34% - IL-5 46% -1.5% TGF- 30% 2% Interferon- 31% - IL-12 16% - PDGF-BB 29% - PMA 39% 0.8% Figure legends for Supplementary Data: Suppl. Figure 1. A-C. htrb genes are expressed in a tissue-specific manner. A Multi-tissue Q-RT-PCR was performed to characterise the expression profile of htrb genes by using "Human Rapid-Scan panel". D. HeLa cells express trb1-3 Total RNA was purified from HeLa cells and expression of the htrb genes was tested by RT-PCR using cDNA (+) or RNA (-) as PCR template Table 1. 10nM final concentration of each cytokines and 50ng/ml PMA were used to stimulate HeLa cells for 4hrs. A. Activation is expressed as relative to the PBS control. B. Those with the capacity to activate AP-1 reporter were studied for the effect of htrb-3. Results are expressed as percent inhibition caused by the cotransfected htrb-3 construct ± Standard Deviation. Abbreviations: IL- Interleukin, bFGF- Basic Fibroblast Growth Factor, EGF- Epidermal Growth Factor, Ins. like GF- Insulin-like Growth Factor, TGF - Transforming Growth Factor Beta-1, M-CSF- Macrophage Colony Stimulating Factor, G-CSF- Granulocyte Colony Stimulating Factor, GM-CSF- Granulocyte Macrophage Colony Stimulating Factor, PDGF-BB- Platelet-derived Growth Factor-BB, LIF- Leukemia Inhibitory Factor, PMA- Tetradecanoylphorbol Acetate