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&'()*++, Journal of Ningxia Medical University !

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1674 - 6309( 2012)

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03 ・ Yt・ P - MEK ?\f]*i^h_`úab??>N\m??? {|}

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1 ( 1. %&"'($4?"$*?Wy, +, 750004;

2.W', +, 750004) 9:: pN de>?y?°Cg?>?Y"°±( VSMCs) ) ERK1/

2 ???`??= MEK( p - MEK) H?? ?I, r?GA??>?y?Il VSMCs {U?I$?H8???g°Z???.qr ?

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24 ??? è??è>?y(( SHR) ?rZ?7, >???è Wistar (( WKY) ?r@:, ?7

5 í(.Zè?.? wJ??8CgH]@?=, z{7D=$8??7C??8i?CgD,úCg?> VSMCs )p - MEK H ??.st SHR ?8i?CgD,úCg VSMCs ?éY??, 8i?Cg\@n?f???-v??8. >PxCg\?, SHR ( p - MEK ・è°±?>5>?? WKY (.SHR

7 p - MEK ?>Hù°>5>? ? WKY 7.s8 >?y?Il?8Cg VSMCs {U?IH$?, ?U;

±n MEK ??=?=¨á. ;

?y;

p - MEK;

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2012 -08 -29 uvwp:}?÷??UV( 210242) 4? ( 2011) IJKL: [\\(

1976 - ) , |, §?, ??, ??^?DE?W? ?.E - mail: caoxm. nxmu@163. com >?y??6??、 y、 ?m??>yF]@ $ ? D { U ? 4.? > ? Y " ° ± (vascular smooth muscle cells, VSMCs) ?é/ ???Zm?{ U?I$??6??>?@(vascular remodeling, VR) 3>?y??H??I$4? [ 1].°8C g3>?y・??H]??>, ? VSMCs bc? kH8???~?7H{U?I?????> ?yH??.`????y?è>?yC???, ???>?y(?8Cg VSMCs )??y?= Mitogen - Activated protein kinase, MAPK) ?K???/??= MEK(p - MEK)H? ?, 8?? p - MEK H????;

>?y?Il? 8Cg VSMCs {U?IH~??, m?GA? c>?y??H8???.

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4 MEK1 / 2IgG ( Ser2211) ?^( Cell Signaling) ;

c?=??X?H O (streptavidin - biotin - peroxidate complex, S - ABC)z {

7 = ? w ? ? ? (USC NLIFE) ;

???? IgG - HRP(*?`:

04 -

15 - 06) ?? KPL ??;

PBS ??#、???p?、 3, 3'- +p4???( 3,

3 - diaminobenzidine DAB) ???, X¨???. 1.

2 NO 1. 2.

1 Z?C???W ?è??è>?y( ( Spontaneously Hypertensive Rats, SHR)

15 í, ?è wistar ((WKY)

15 í(??.b?~Z?C? ¨???) .(h?z??Cg???4??y ??}.Z???, ?¨(?ó?5 SP - F P? ?), ]?、 ??3:、 ??F?;

??>Gz?? BL - 420E + ???UZ?yF(????'?¨ ???) CI???í(H?Cgy;

? SHR ( D WKY (?8?-7, ¤

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24 ??7, ?

7 5 í.?:Z?87, ?Z?(ó??\e? ?z??Y? 3% =g???( 50mg・ kg -

1 )??, ?^CgI>u?+k?W???????Cg y( MAP) .jc$?é?5 DEPC ?W?H 4% 1∶ 1000))@l 48h.??? ?w?、 d8, Z?5?sZ?. 1. 2.

2 ?8CgZè?.?w Zè?.?w? " ??'O"Zè?.r??2w, !?°±r_ ?w.J?Z?(?8i?Cgn?Zè?. 3?f?b???¨;

Cg>?¨W???.=$ ?;

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3 z{7=O i? S - ABC O.??V? ???m??d?N?, w??d8.S - ABC O ??aHH_?X`? ・è@:、 PBS ?dG??r?è@:, DAB ?w. ??J????éz{7=d8, f?0j

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20 ",úCg) , p - MEK1 /

2 ?wm± ?h_4wb4(wü??r・è°±.??? ÷・è°±H??D8? 100% . 1.

3 FL$NO ? SPSS 11.

5 FLIJ??? ?FL, L?í?m?? ± XùK(x±s)??, ? 7???i? t ??.P < 0.

05 rK?¨FL$ 23.

2 st 2.

1 ?y?= Z???(F?、 ?C??, W C?F?_ê.SHR (H MAP 1f???>? ???-, WKY ( MAP 1H?>ù°?5 SHR (,

16 D

24 ?? SHR 7??>5>?? WKY 7( P <

0 01) , ?÷ 1. ?

1 ìk*iú0_`](MAP)N~`stê? 2.

2 ?8i?CgD,úCgH]@?= ?7 (?8i?CgD,úCg VSMCs °±?Vf a???>?=Y??, ??? SHR D WKY 7? K?WFL$23(P > 0. 05) , ?÷ 2A ~ B;

?? 'OZè?.?wL??8i?Cg¨??H? .=$?, ?÷ 2C( ! 2) , Q`Z?)b( G ?w c5_, F!?L?_(w;

,úCg>?à¨? ?HZè?., FLJ?|?]@H?=.SHR

7 8i?Cg\@n??1f???-v??8, WKY 7?=Y??, f?1i

16、

24 ?? SHR

7 ???5 WKY 7(( P < 0. 05) , ?÷ 2D. ? 2A ^hc?_` VSMC ?pê? ? 2B ^Ud_` VSMC ?pê? ? 2D ^hc?_`?bc8êe 2.

3 ?8i?Cg VSMCs ) p - MEK H?? faZ?(???>, SHR (?8i?CgD ,úCg VSMCs H p - MEK ・è}N>;

SHR (  p - MEK ・è°±?>5\>??H WKY ( , ?8i?Cgi