PDF《Anti-TIMP4 antibody ab38987》

产 产品名称 品名称 Anti-TIMP4抗体 描述 描述 兔多克隆抗体to TIMP4 宿主 宿主 Rabbit 特异性 特异性 Ab38987 recognises the carboxiterminal region of TIMP4.

It does not react with other TIMPs. 经测试应 经测试应用 用 适用于 适用于: WB 种属反 种属反应 应性 性 与反 与反应 应: Rat, Sheep, Human 免疫原 免疫原 Synthetic peptide based on the carboxyterminal region of human TIMP4. 参阅Abcam的专有抗源 政策 (Peptide available as ab41155.) 阳性 阳性对 对照 照Human TIMP4, cell media from human colorectal adenosarcoma and rat heart. 形式 形式 Liquid 存放 存放说 说明 明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. 存 存储 储溶液 溶液 pH: 7.40 Preservative: 0.05% Sodium azide Constituent: 2.9% Sodium chloride 纯 纯度 度Immunogen affinity purified 克隆 克隆 多克隆 同种型 同种型 IgG Product datasheet Anti-TIMP4 antibody ab38987

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1 References

1 图像 概述 概述 性能 性能 应 应用 用Our Abpromise guarantee covers the use of ab38987 in the following tested applications. The application notes include recommended starting dilutions;

optimal dilutions/concentrations should be determined by the end user. 应应用用Ab评论 评论 说 说明 明1功能 功能 Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. 组织 组织特异性 特异性 Abundant in heart and present at low levels in many other tissues. 序列相似性 序列相似性 Belongs to the protease inhibitor I35 (TIMP) family. Contains

1 NTR domain. 细 细胞定位 胞定位 Secreted. Western blot - Anti-TIMP4 antibody (ab38987) All lanes : Anti-TIMP4 antibody (ab38987) at 1/1000 dilution Lane

1 : human TIMP4 Lane

2 : cell media from human colorectal adenosarcoma Lane

3 : rat heart Predicted band size:

26 kDa Observed band size: 25,45,66,97 kDa why is the actual band size different from the predicted? Additional bands at:

20 kDa,

23 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands. WB 1/1000 - 1/5000. Detects a band of approximately

23 kDa (predicted molecular weight:

26 kDa). Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight:

26 kDa. When used against the reduced protein, ab38987 identifies bands at

23 and

29 kDa, representing the unglycosylated and glycosylated forms of TIMP4 respectively. Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. 应应用用Ab评论 评论 说 说明 明靶靶标 标图图片 片Please note: All products are "FOR RESEARCH USEONLY. NOTFOR USEIN DIAGNOSTIC PROCEDURES" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet

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12 months from date of delivery Response to your inquiry within

24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit https://www.abcam.cn/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3