PDF《ABfinity™ TIMP4 Recombinant Rabbit》

ABfinity? TIMP4 Recombinant Rabbit Monoclonal Antibody Store at 2°C to 8°C (short-term), or C20°C (long-term) Catalog Number

701317 Pub.

No. MAN0007534 Rev. 1.00 Clonality: Monoclonal Quantity:

100 μg Volume:

200 μL Concentration: 0.5 mg/mL Host/Class: Rabbit IgG Reactivity: Human TIMP4 Predicted Reactivity: Human Product Description Tissue inhibitor of metalloproteinase

4 (TIMP4) is a member of the tissue inhibitor of metalloproteinases gene family. It functions to inhibit the activity of matrix metalloproteinases (MMP) which degrade components of the extracellular matrix. TIMP4 binds irreversibly to zinc-dependent MMPs, and inactivates them by binding to their catalytic zinc cofactor. Other functions of TIMP4 include platelet recruitment and aggregation, and nervous system development. In humans, the gene encoding TIMP4 is located on chromosome 3. Product Specifications Immunogen: Folded protein domain corresponding to amino acids of Human TIMP4 Apparent MW: ~

25 kDa Gene ID:

7079 Protein Accession No.: Q99727 Sequence Identity: Human Isotype: IgG1 Lot: See product label Product Applications Application Species Test Material Concentration Western blotting Human MDA-MB-231 cells 2C3 μg/mL Indirect ELISA Human HeLa lysate 0.5 * 10-3 to

10 ?g/mL Immunocyto- chemistry Human U2OS cells

1 ?g/mL Flow cytometry Human HeLa cells

1 ?g/mL for

1 * 10-6 cells Storage and handling Store the antibody at 2°C to 8°C for up to

1 month, or C20°C for long storage. Avoid repeated freezing and thawing. Stability When stored as instructed, expires one year from date of receipt unless otherwise indicated on product label. Figure

1 Western blot analysis of ABfinity? TIMP4 Recombinant Rabbit Monoclonal Antibody (Cat. no. 701317). Western blot analysis was performed on MBA-MB-231 whole cell extracts. Endogenous level of TIMP4 was detected at ~25 kDa using ABfinity? TIMP4 Recombinant Rabbit Monoclonal Antibody at a concentration of

2 μg/mL. The blot was developed using enhanced chemiluminescence (ECL) method. Storage Buffer Phosphate buffered saline (PBS) with 0.09% sodium azide. CAUTION! Sodium azide is extremely toxic and may react with lead and copper plumbing to form highly explosive metal azides. Properly dispose of solutions containing sodium azide. Read the Safety Data Sheet (SDS) and follow the handling instructions. Wear appropriate protective eyewear, clothing and gloves. SDSs are available at www.lifetechnologies.com/ support. For Research Use Only. Not for use in diagnostic procedures. Manufacturing Site ?

7335 Executive Way ? Frederick ? MD

21704 ? E-mail: [email protected] Limited product warranty Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale found on Life Technologies' website at www.lifetechnologies.com/termsandconditions. If you have any questions, please contact Life Technologies at www.lifetechnologies.com/support. Figure

2 Indirect ELISA of ABfinity? TIMP4 Recombinant Rabbit Monoclonal Antibody (Cat. no. 701317). Indirect ELISA was performed using various dilutions of ABfinity? TIMP4 Recombinant Rabbit Monoclonal Antibody to detect endogenous TIMP4 in HeLa lysate, coated onto the plate. A non-linear regression analysis was performed (4 PL), LOD, and LOQ for the antibody was determined. Figure

3 Immunocytochemistry analysis of ABfinity? TIMP4 Recombinant Rabbit Monoclonal Antibody (Cat. no. 701317). Immunocytochemistry analysis of U20S cells stained with ABfinity? TIMP4 Recombinant Rabbit Monoclonal Antibody using a: Alexa Fluor?