100 pUNO1-mTIMP4 (3879 bp) BsrS2 SV40 p(A) (h) ?globin p(A) pMB1 ori hCMV enh hCMV prom EM7 hEF1-HTLV prom mTIMP4 SgfI (6) PvuI (7) MfeI (82) Psp1406I (203) HindIII (245) Bsu36I (291) NgoMIV (441) KasI (535) AgeI (552) SphI (560) FspI (663) MscI (912) BbsI (937) EcoO109I (974) XcmI (1017) PstI (1173) BspEI (1202) NheI (1261) MscI (1267) HpaI (1399) MfeI (1410) EcoRI (1495) SapI (1677) SspI (1734) SwaI (1748) EcoO109I (1809) SacI (2009) StuI (2173) XmnI (2315) BbsI (2319) BspHI (2323) AseI (2381) SacI (2438) SpeI (2536) SnaBI (2664) NdeI (2769) SdaI (2947) PstI (2948) PacI (2955) BspLU11I (2965) ApaLI (3279) PacI (3695) SwaI (3704) NotI (3714) EagI (3715) 15L15v40 GGATCTGCGATCGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACGGGTGCCTA GAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCC GTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGCTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGAGGCC GCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCCTCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACC GGGCCTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTTTGCCTGACCCTGCTTGCTCAACTCTACGTCTTTGTTTCGTTT TCTGTTCTGCGCCGTTACAGATCCAAGCTGTGACCGGCGCCTACCTGAGATCACCGGTCAGCATGCCCTGGAGTCCCCTGGCTGCGCTGAGCTGGGCGCT GGTGCTGAGGCTGCTGGCTTTGCTGTGGCCCCCTGGACGGGGTGAGGCGTGCAGCTGTGCGCCTGCGCACCCCCAGCAGCACTTCTGCCACTCGGCTCTA GTGATACGGGCCAAAATATCCAGTGAGAAGGTAGTCCCTGCCAGCAAAGACCCTGCTGACACTCAAAAACTGATCCGGTATGAAATCAAACAGATAAAGA TGTTTAAAGGGTTCGAGAAGGCCAAGGATATTCAGTATGTCTACACGCCATTTGACTCTTCCCTCTGTGGTGTGAAGCTAGAAACCAACAGTCACAAGCA GTATCTTTTGACTGGCCAGATTCTCAGTGATGGAAAAGTCTTCATCCATCTGTGCAACTACATTGAGCCCTGGGAGGACCTGTCCTTGGTGCAGAGGGAG AGCCTGAATCATCACTACCACCAGAACTGTGGCTGCCAAATCACCACTTGCTATGCAGTGCCATGTACCATCTCAGCCCCCAATGAGTGTCTCTGGACGG ACTGGCTGCTGGAACGGAAGCTCTATGGGTACCAGGCCCAGCACTATGTCTGCATGAAGCATGTTGATGGCATCTGCAGCTGGTACCGAGGCCACCTACA CCTCCGGAAGGAGTACGTTGACATCATCCAGCCCTAGTAGGGACCAGGAACCACATCCTTGGCTAGCTGGCCAGACATGATAAGATACATTGATGAGTTT GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAG TTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGAATT CTAAAATACAGCATAGCAAAACTTTAACCTCCAAATCAAGCCTCTACTTGAATCCTTTTCTGAGGGATGAATAAGGCATAGGCATCAGGGGCTGTTGCCA ATGTGCATTAGCTGTTTGCAGCCTCACCTTCTTTCATGGAGTTTAAGATATAGTGTATTTTCCCAAGGTTTGAACTAGCTCTTCATTTCTTTATGTTTTA AATGCACTGACCTCCCACATTCCCTTTTTAGTAAAATATTCAGAAATAATTTAAATACATCATTGCAATGAAAATAAATGTTTTTTATTAGGCAGAATCC AGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTCT AGCTTTAGTTCCTGGTGTACTTGAGGGGGATGAGTTCCTCAATGGTGGTTTTGACCAGCTTGCCATTCATCTCAATGAGCACAAAGCAGTCAGGAGCATA GTCAGAGATGAGCTCTCTGCACATGCCACAGGGGCTGACCACCCTGATGGATCTGTCCACCTCATCAGAGTAGGGGTGCCTGACAGCCACAATGGTGTCA AAGTCCTTCTGCCCGTTGCTCACAGCAGACCCAATGGCAATGGCTTCAGCACAGACAGTGACCCTGCCAATGTAGGCCTCAATGTGGACAGCAGAGATGA TCTCCCCAGTCTTGGTCCTGATGGCCGCCCCGACATGGTGCTTGTTGTCCTCATAGAGCATGGTGATCTTCTCAGTGGCGACCTCCACCAGCTCCAGATC CTGCTGAGAGATGTTGAAGGTCTTCATGATGGCCCTCCTATAGTGAGTCGTATTATACTATGCCGATATACTATGCCGATGATTAATTGTCAAAACAGCG ? N R T Y K L P I L E E I T T K V L K G N M E I L V F C D P A Y D S I L E R C M G C P S V V R I S R D V E D S Y P H R V A V I T D F D K Q G N S V A S G I A I A E A C V T V R G I Y A E I H V A S I I E G T K T R I A A G V H H K N D E Y L M T I K E T A V E V L E L D Q Q S I N F T K M M P W S P L A A L S W A L V L R L L A L L W P P G R G E A C S C A P A H P Q Q H F C H S A L V I R A K I S S E K V V P A S K D P A D T Q K L I R Y E I K Q I K M F K G F E K A K D I Q Y V Y T P F D S S L C G V K L E T N S H K Q Y L L T G Q I L S D G K V F I H L C N Y I E P W E D L S L V Q R E S L N H H Y H Q N C G C Q I T T C Y A V P C T I S A P N E C L W T D W L L E R K L Y G Y Q A Q H Y V C M K H V D G I C S W Y R G H L H L R K E Y V D I I Q P ? SgfI (6) PvuI (7) MfeI (82) Psp1406I (203) HindIII (245) Bsu36I (291) NgoMIV (441) KasI (535) AgeI (552) SphI (560) FspI (663) MscI (912) BbsI (937) EcoO109I (974) XcmI (1017) PstI (1173) BspEI (1202) NheI (1261) MscI (1267) HpaI (1399) MfeI (1410) EcoRI (1495) SapI (1677) SspI (1734) SwaI (1748) EcoO109I (1809) SacI (2009) StuI (2173) XmnI (2315) BbsI (2319) BspHI (2323) AseI (2381)