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PAA014Ra01 Polyclonal Antibody to Bone Morphogenetic Protein

4 (BMP4) Organism Species: Rattus norvegicus (Rat) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: BMP4, Rat Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200?g/mL UOM: 100?g [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant BMP4 (Pro28~Glu183) with N-terminal His-Tag expressed in E.coli. Accession No.: RPA014Ra01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against BMP4. It has been selected for its ability to recognize BMP4 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant BMP4 (Pro28~Glu183) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4o C for frequent use. Stored at -20o C to -80o C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] Used in Western Blot, Sample: Recombinant BMP4, Rat Used in DAB staining on fromalin fixed paraffin- embedded stomach tissue