PDF《BOSTER》

BOSTER 博士德生物 Product Information 地址:武汉市光谷大道特一号国际企业中心聚贤楼 B 座5楼

电话:027-67845390 800-880-8748 传真:(027)67845396

邮箱:bosterr@188.

com

网址:www.boster.com.cn BOSTER 在/平/凡/中/创/造/奇/迹BOSTER Rabbit Anti―CD3E /CD3 epsilon CATALOG No. PB0112 LOT No. ZP652BP52 Concentration 200μg/ml SIZE 0.1ml;

0.2ml Bckground CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Preparation Produced in rabbits immunized E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon. CD3 epsilon specific antibody was purified by recombinant protein affinity chromatography. Specificity It reacts specifically with CD3 epsilon of human, mouse and rat origin in immunostaining and western blotting. No cross-reactivity with other members of the family. Application Western blotting: 1:300-3000 Immunocytochemistry in fixed cells: 1:100-500 Immunohistochemistry in frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-400 Immunofluorescence: 1:50-200 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,for

20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end users. FORMAT Immunoaffinity purified rabbit IgG. Contents 0.01 M PBS, 30% glycerol. 0.02% NaN3. Storage For continuous use store at 4?C for six months. For long term storage store at-20° C in small aliquots to prevent freeze-thaw cycles. Reference 1. Clevers HC, Dunlap S, Wileman TE, Terhorst C (November 1988)."Human CD3-epsilon gene contains three miniexons and is transcribed from a non-TATA promoter". Proc. Natl. Acad. Sci. U.S.A. 85(21): 8156C60. 2. "Entrez Gene: CD3E CD3e molecule, epsilon (CD3-TCR complex)" 3. Gold DP, Puck JM, Pettey CL, Cho M, Coligan J, Woody JN, Terhorst C (1986). "Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex". Nature

321 (6068): 431C4. BOSTER 博士德生物 Product Information 地址:武汉市光谷大道特一号国际企业中心聚贤楼 B 座5楼

电话:027-67845390 800-880-8748 传真:(027)67845396

邮箱:[email protected]

网址:www.boster.com.cn BOSTER 在/平/凡/中/创/造/奇/迹BOSTER IH(P);

IC;

IF relevant reagents 博士德提供一系列免疫组织/细胞化学使用的二抗试剂盒: 用于冰冻切片的免疫组化时,使用 SA1027(小鼠 IgG)/SA1028(兔IgG) ;

用于石蜡切片的免疫组化时,使用三步法 SA1021(小鼠 IgG)/SA1022(兔IgG) 或超敏二步法 SV0001(小鼠 IgG)/SV0002(兔IgG) ;

此外博士德还提供其它标记物(包括 FITC,Cy3 和碱性磷酸酶)的免疫组化试剂盒及其 他全系列产品,例如: 4%多聚甲醛,货号:AR1068(不含 DEPC)/AR1069(含DEPC);

DAB 显色剂,货号:AR1022;

苏木素,货号:AR0005;

多聚赖氨酸,货号:AR0003;

抗体稀释液,货号:AR1016;

PBS 缓冲液,货号:AR0030;

枸橼酸盐缓冲液,货号:AR0024;

防脱片载玻片,货号:AR1065;

Western Blotting relevant reagents 博士德提供一系列 Western Blotting 实验使用的辅助试剂,例如: WB 专用一抗二抗稀释液,货号:AR1017;

HRP-羊抗兔 IgG,货号:BA1054;

HRP-羊抗小鼠 IgG,货号:BA1050;

蛋白干粉,货号:AR0104;

BCA 蛋白定量试剂盒,货号: AR0146;

凝胶配置试剂盒,货号:AR0138;

PMSF-1ml (100mmoL/L) , 货号: AR1178;

丽春红染色液(10X), 货号: AR0142;

超敏 ECL 化学发光即用底物,货号:AR1171;

显影定影试剂盒,货号:AR0132;

此外各种蛋白抽提试剂盒,酶抑制剂,NC 膜,Marker,上样/电泳/漂洗/转印缓冲液, 膜再生液,X 光背景去除液,WB 专用 DAB 显色试剂盒等系列产品均有现货提供.