PDF《ABSTRACT》

Pakistan Vet.

J., 2007, 27(1): 29-34.

29 EFFECT OF BULL AND SPERM PREPARATION METHOD ON IN VITRO FERTILIZATION OF BUFFALO OOCYTES H. JAMIL, H. A. SAMAD, Z. I. QURESHI, N. REHMAN AND L. A. LODHI Department of Animal Reproduction, University of Agriculture, Faisalabad, Pakistan ABSTRACT The present study was designed to compare fertilization rates following oocyte exposure to spermatozoa from different buffalo bulls, using three sperm preparation methods i.e. percoll density gradient, swim-up (modified Ca2 free Tyrode's medium and TALP medium) and sodium citrate washing prior to co-incubation with oocytes. Buffalo oocytes were aspirated from ovarian follicles within 1.5 to

2 hours after slaughter. They were matured in TCM-199 supplemented with 20% oestrus buffalo serum drops under mineral oil in CO2 incubator at 39°C for

24 hours. Matured oocytes were transferred to the fertilization droplets and inseminated with 1x106 capacitated sperms prepared by different experimental methods. Data collected on recovered sperm motility immediately after treatment and

24 hours after incubation (at 37°C) and cleavage rate of co-incubated oocytes were subjected to statistical analysis. The percentage of motile spermatozoa was significantly higher (P