PDF《和DXS7423 基因座遗传多态性》

收稿日期:2013-10-18;

定稿日期:2014-05-23 基金项目:广西自然科学基金项目(2010GXNSFA013164) 作者简介:周丽宁(1970-),女,广西壮族自治区梧州藤县人,广西医科大学副教授,硕士,主要从事人类学及群体遗传学 研究.

E-mail:[email protected] 通讯作者:覃耀春(1967-),男,广西壮族自治区贵港市人,硕士,E-mail:[email protected] Citation: Zhou LN, Gong JC, Lun J, et al. Genetic of DXS7133, DXS8378, DXS6789 and DXS7423 in the Maonan Ethnic Group from Guangxi Province[J]. Acta Anthropologica Sinica, 2015, 34(3): 397-403 广西毛南族 DXS

7133、DXS

8378、DXS6789 和DXS7423 基因座遗传多态性 周丽宁

1 ,龚继春

1 ,伦军1 ,邓琼英

1 ,覃耀春

2 1.广西医科大学人体解剖学教研室,南宁 530021;

2.广西医科大学公共卫生学院,南宁

530021 摘要:采用 PCR-STR 及基因分型技术,对广西毛南族

167 名(女57,男110)健康无关个体

4 个X-STR 基因座(DXS

7133、DXS

8378、DXS6789 和DXS7423)的遗传多态性进行研究.结果显示

4 个X-STR 基因座分别检出

4、

5、

9、3 个等位基因和

5、

9、

18、5 种基因型,4 个X-STR 基因座女性的基 因型频率分布均符合 Hardy-Weinberg 平衡定律(P>

0.05).群体遗传多态性指标为:多态信息含量(PIC) 0.

9611、男性个体识别率(DPmale)0.

9771、女性个体识别率(DPfemale)0.

9980、父-母-女三联体非父排 除率(MECtrio)0.

9611、父-女二联体非父排除率(MECduo)0.8821,显示上述

4 个X-STR 基因座均具 有较高多态性,在法医学个人识别、亲权鉴定及群体遗传学研究中有重要应用价值,同时也为人类群体 遗传学、法医学等研究提供了广西毛南族群体 X-STR 基因座的基础数据,丰富了中华民族基因数据库. 关键词 : X 染色体;

短串联重复序列;

遗传多态性;

遗传距离;

广西毛南族 中图法分类号:Q987;

文献标识码:A;

文章编号:1000-3193(2015)03-0397-07 Genetic of DXS7133, DXS8378, DXS6789 and DXS7423 in the Maonan Ethnic Group from Guangxi Province ZHOU Lining1 , GONG Jichun1 , LUN Jun1 , DENG Qiongying1 , QIN Yaochun2 1. Department of Anatomy, Guangxi Medical University, Nanning 530021;

2. College of Public Health, Guangxi Medical University, Nanning

530021 Abstract: To determine the genetic polymorphism of four X-STR loci among

167 unrelated individuals (57 female,

110 male) for Maonan groups from Guangxi Province, DXS7133, DXS8378, DXS6789 and DXS7423 were genotyped by multiplex PCR and Genscan. The numbers of alleles in the four X-STR loci were 4, 5,

9 and 3, respectively;

the numbers of genotypes in the four loci were 5, 9,

18 and 5, respectively. The genotype frequencies in females were in accordance with Hardy-Weinberg equilibrium (P>

0.05). The combined polymorphism DOI: 10.16359/j.cnki.cn11-1963/q.2015.0040 ? ?

398 ?

34 卷information content (PIC) was 0.9611, power of discrimination (DP) was 0.9771 in males and 0.0.9980 in females, and the combined mean exclusion chance (MEC) was 0.0.9611 in the trio cases and 0.8821 in the duo cases. The

4 X-STR loci were highly polymorphic in Guangxi Maonan population and it is powerful for individual identification, paternity testing and population genetics. As well as the human population genetics and forensic science research offers Guangxi Maonan group X-STR loci of basic data, enrich the genetic database of the Chinese nation. Key words: X-chromosome;

Short tandem repeats;

Genetic polymorphism;