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1 SUPPLEMENTARYINFORMATION doi:10.1038/nature11651

1 Supplementary Information: F-GmSUB1-cDNA cccaaagtgttgtcagtgttcgagaaccgtgggagaaagctacacaccactcgatcatgg

360 V E-GmSUB1-cDNA cccaaagtgttgtcagcgttcgagaaccgtgggagaaagctacacaccactcgatcatgg

360 A W-GmSUB1-cDNA cccaaagtgttgtcagtgttcgagaaccgtgggagaaagctacacaccactcgatcatgg

360 V F-GmSUB1-cDNA cttggtaacgatattaccttcaagggggaaagcttatcagctacaaaattggcacacaag

1140 F E-GmSUB1-cDNA cttggtaacgatattaccttcaagggggaaagcttatcagctacaaaattggcacacaag

1140 F W-GmSUB1-cDNA cttggtaacgatattaccttgaagggggaaagcttatcagctacaaaattggcacacaag

1140 L F-GmSUB1-cDNA tataaatgccgcaagaaatttagtctcctcaacctgaactatccctcaatcacagtccca

2040 L E-GmSUB1-cDNA tataaatgccgcaagaaatttagtctcctcaacctgaactatcccctaatcacagtccca

2040 S W-GmSUB1-cDNA tataaatgccgcaagaaatttagtctcctcaacctgaactatccctcaatcacagtccca

2040 L F-GmSUB1-cDNA tacattgctcatgttcagaacccgtatggaatcaccgtttctgtgaagccaagcatcttg

2160 Q E-GmSUB1-cDNA tacattgctcatgttcaaaacccgtatggaatcaccgtttctgtgaagccaagcatcttg

2160 Q W-GmSUB1-cDNA tacattgctcatgttcaaaacccgtatggaatcaccgtttctgtgaagccaagcatcttg

2160 Q Figure S1. Sequence analysis of the GmSUB1 gene mapped to the Rhg4 locus. A comparison of GmSUB1 cDNA and predicted protein sequences between the SCN-resistant soybean cv. Forrest (F) and the SCN-susceptible cvs. Essex (E) and Williams

82 (W). The five nucleotide differences are highlighted in gray and the corresponding amino acids in black. SUPPLEMENTARY INFORMATION

2 | W W W. N A T U R E . C O M / N A T U R E RESEARCH

2 a) F-GmSHMT-Promoter caatggcaccaatgcccaatgggagatttaagtcaagcccaacatcaacctctgaaatta

60 E-GmSHMT-Promoter caatggcaccaatgcccaatgggagatttaagtcaagcccaacatcaacctctgaaatta

60 W-GmSHMT-Promoter caatggcaccaatgcccaatgggagatttaagtcaagcccaacatcaacctctgaaatta

60 F-GmSHMT-Promoter tgaattatgaaattaaaatgcttcctagtaagtgaactagttgcatctcatttatatcat

120 E-GmSHMT-Promoter tgaattatgaaattaaaatgcttcctagtaagtgaactagttgcatctcatttatatcat

120 W-GmSHMT-Promoter tgaattatgaaattaaaatgcttcctagtaagtgaactagttgcatctcatttatatcat

120 F-GmSHMT-Promoter aaatttcgaactacgactttcttggccatgttagtaaagtttgggggattgttcaaaatt

180 E-GmSHMT-Promoter aaatttcgaactacgactttcttggccatgttagtaaagtttgggggattgttcaaaatt

180 W-GmSHMT-Promoter aaatttcgaactacgactttcttggccatgttagtaaagtttgggggattgttcaaaatt

180 F-GmSHMT-Promoter ggtggagtggttcagcttaatctccaaattatttgttctaagttgttttggtaggcaggt

240 E-GmSHMT-Promoter ggtggagtggttcagcttaatctccaaattatttgttctaagttgttttggtaggcaggt

240 W-GmSHMT-Promoter ggtggagtggttcagcttaatctccaaattatttgttctaagttgttttggtaggcaggt

240 F-GmSHMT-Promoter ttaattttttcctgatcctgggaaaaaaattattgataccatattaacatctcttgacga

300 E-GmSHMT-Promoter ttaattttttcctgatcctgggaaaaaaattattgataccatattaacatctcttgacga

300 W-GmSHMT-Promoter ttaattttttcctgatcctgggaaaaaaattattgataccatattaacatctcttgacga

300 F-GmSHMT-Promoter tgctacgagatttctcatgattatagaactgagtagggtggcttaaaaggttttatttta

360 E-GmSHMT-Promoter tgctacgagatttctcatgattatagaactgagtagggtggcttaaaaggttttatttta

360 W-GmSHMT-Promoter tgctacgagatttctcatgattatagaactgagtagggtggcttaaaaggttttatttta

360 F-GmSHMT-Promoter aatataatttcaccacattgaattgggtattagtaaactggttactggtatgcctgtaaa

420 E-GmSHMT-Promoter aatataatttcaccacattgaattgggtattagtaaactggttactggtatgcctgtaaa

420 W-GmSHMT-Promoter aatataatttcaccacattgaattgggtattagtaaactggttactggtatgcctgtaaa

420 F-GmSHMT-Promoter gtggacaatgataaatgtttttatagaagttggtatggattttaaaatagctcatgtata

480 E-GmSHMT-Promoter gtggacaatgataaatgtttttatagaagttggtatggattttaaaatagctcatgtata

480 W-GmSHMT-Promoter gtggacaatgataaatgtttttatagaagttggtatggattttaaaatagctcatgtata