PDF《天津和唐山小豆地方品种遗传多样性分析》

植物遗传资源学报2011,12(5):679―685 Joumal of Plant Genetic Resources 天津和唐山小豆地方品种遗传多样性分析 刘振兴1,程须珍2,王丽侠2,王素华2,周桂梅1,陈健1 ('

河北省唐山市农业科学研究院,唐山063000;

2中国农业科学院作物科学研究所,北京100081) 摘要:利用SSR分子标记对唐山、天津的96个红小豆品种(按品种地理来源划分为14个品种群)进行遗传多样性分析, 结果表明,197对引物中共有89对的扩增谱带清晰稳定.

共扩增出285条带,多态性条带百分率(PPB)达100%,经Popgene32 软件分析,遵化品种资源的遗传多样性水平最高[PPB=85.39%,I(信息指数)=0.567],其次为玉田和迁安的品种资源,而在 天津红小豆资源中,除静海县(排位第5)外,遗传多样性水平相对较小,其中宁河的遗传多样性水平最低(PPB=22,47%,I= 0.1575).聚类分析表明,96个品种可分为三个组群,唐山红小豆组群中包含了天津静海小豆;

天津红小豆中的大港品种资源 单独为一组群;

其他天津红小豆为第二大组群.本研究可为地方品种的保存及杂交组配提供参考. 关键词:小豆;

地方品种;

SSR;

遗传多样性 Genetic Diversity of Adzuki Bean Landrace in Tianjin and Tangshan LIU Zhen.xin91,CHENG Xu.zhen2,WANG Li-xia2, WANG Su.hua2,ZHOU Gui―meil,CHEN Jianl (1 Tangshan Academy of Agricultural Sciences.Hebei Provinces,Tangshan 063001;

2Institute ofGrop Sciences,Chinese Academy ofAgricultural Sciences,BeOing 100081) Abstract:The genetic diversity of

96 adzuki bean landraces in Tangshan and Tianjin was investigated by SSR molecular markers.These materials can be divided into

14 variety group in terms of their geographical origins.89 out of

1 97 pairs of primers were detected producing

285 bands,with total percentage of polymorphic bands(PPB) was

1 00%.The genetic diversity of'

Zunhua'

variety group was the highest and that of Yutian and Qianan wast second higest.A11.1andraces from Tianjin excluding Jinghai variety group has relatively low genetic diversity and the lowest PPB with 22.47%can be found in Ninghe polpulation.total of

96 accessions are divided into three groups by genetic similarities.Jinghai varety group from Tianjin was included by Tangshan adzuki bean and Da・ gang variety group from Tianjin was one separate group.The rest accessions of Tianjin adzuki bean froms another group.So reference for landraces conservation and selection of diverse parental combinations can be provided by this research. Key words:Adzuki bean;

Landrace;

SSR;

Genetic diversity 中国是小豆的起源地,资源拥有量占世界第一 位….但小豆一直被认为是 小作物 ,大多种植在 沟边地头及山地丘陵等瘠薄地,品种也较杂乱.尽 管这些年来小豆育种成就颇为显著,但新品种推广 面积并不大,生产上主要以地方品种为主.唐山红 小豆、天津红小豆是我国传统的名优地方品种,唐山 红、天津红是指在唐山、天津地区种植的红小豆品种 的总称,在国内外享有盛誉,因其具有较强的抗逆 性,作为亲本,通过杂交等手段已培育出多个高产优 质红小豆新品种旧1,因此对唐山、天津等红小豆地 方品种的搜集、保存及其利用有重要意义. 遗传多样性主要指物种内不同品种的遗传变 异.遗传多样性的研究,有助于了解品种的亲缘关 系和群体结构等信息,对作物品种资源的保护、利用 收稿日期:2011・05・19 修回日期:2011-05―26 基金项目:国家食用豆行业科研(nyhyzx07―017);