PDF《Functional Angiopoietin-2 Antibody,》

9765 Clairemont Mesa Blvd, Suite C San Diego, CA

92124 Tel: 858.

875.1900 Fax: 858.622.0609 Functional Angiopoietin-2 Antibody, mAb (recombinant) (blocking) Catalog # ADP0006 Specification Functional Angiopoietin-2 Antibody, mAb (recombinant) (blocking) - Product Information Application E Primary Accession O35608 Reactivity Human, Mouse Host CHO Cells Clonality Monoclonal Isotype Mouse IgG2bλ. Gene Source Human Application Note ,E,Functional Appli cational,Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50= 50-60ng/ml (for 10ng/ml of mouse Angiopoieti n-2) ,Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50= 8-12ng/ml (for 10ng/ml of human Angiopoieti n-2) ,ND50 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2). Clone Names Angy-2-1 Calculated MW

56576 Description anti-Angiopoietin- 2, mAb (rec.) (blocking) (Angy-2-1) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the mouse lgG2b Fc domain. Functional Angiopoietin-2 Antibody, mAb (recombinant) (blocking) - Additional Information Gene ID

11601 Other Names Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1). Methods:Tie-2 (h):Fc was coated on an ELISA plate at

1 ?g/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) or an unrelated mAb (recombinant) (Control) were added (starting at

40 ?g/ml with a twofold serial dilution) together with 20ng/ ?l of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP). Functional Angiopoietin-2 Antibody, mAb (recombinant) (blocking) - Background Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the Ang-2;

Ang2;

Angpt2;

Agpt2 Target/Specificity Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1. Format Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. Reconstitution & Storage Stable for at least

1 year after receipt when stored at -20°C. Precautions Functional Angiopoietin-2 Antibody, mAb (recombinant) (blocking) is for research use only and not for use in diagnostic or therapeutic procedures. Functional Angiopoietin-2 Antibody, mAb (recombinant) (blocking) - Protein Information Name Angpt2 Synonyms Agpt2 Function Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity). Cellular Location Secreted. Tissue Location Expressed only at sites of vascular remodeling Functional Angiopoietin-2 Antibody, mAb (recombinant) (blocking) - Protocols Provided below are standard protocols that you may find useful for product applications. ? Western Blot ? Blocking Peptides ? Dot Blot ? Immunohistochemistry ? Immunofluorescence ? Immunoprecipitation ? Flow Cytomety ? Cell Culture human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. Powered by TCPDF (www.tcpdf.org)