PDF《Anti-Thrombopoietin》

Anti-Thrombopoietin produced in goat, affinity isolated antibody Catalog Number T4930 Product Description Anti-Thrombopoietin (TPO) is developed in goats using as the immunogen recombinant mouse TPO, expressed in NOS cells.

The product is affinity isolated. TPO, the ligand for the receptor encoded by the c-Mpl protooncogene, acts as a stimulator of the development of megakaryocyte precursors of platelets. Similar to erythropoietin, TPO leads to an increase in the number of circulating platelets. It affects the entire thrombopoietic process, with stronger effects in the later stages. Other thrombopoietic cytokines include stem cell factor (SCF), IL-3, IL-6, and IL-11. TPO is an ~35 kDa polypeptide of

335 amino acids. However, due to glycosylation, the protein has an apparent MW of

75 kDa in SDS-PAGE. The precursor form of TPO consists of

356 amino acids. To generate the mature TPO (335 amino acids), the precursor cleaves a

21 amino acid signal peptide. Human, mouse and dog TPO show 69-75% amino acid homology. Reagent Supplied lyophilized from phosphate buffered saline, pH 7.4, containing 5% trehalose. Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Storage/Stability Prior to reconstitution, store at C20 °C. Reconstituted product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at C20 °C. Avoid repeated freezing and thawing. Preparation Instructions To one vial of lyophilized powder, add

1 ml of 0.2 ?m filtered PBS to produce a

100 ?g/ml stock solution. If aseptic technique is used, no further filtration should be needed for use in cell culture environments. Product Profile Anti-Thrombopoietin is tested for its ability to neutralize the biological activity of recombinant mouse TPO on the human cell line, MO7e.1 The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of the bioactivity of recombinant mouse TPO, when recombinant mouse TPO is present at a concentration just high enough to elicit a maximum response. The antibody may also be used in immunoblotting and ELISA. By ELISA, the antibody shows