PDF《人尿微量白蛋白（MAU;ALB）酶联免疫分析（ELISA）》

ALB in the samples is then determined by comparing the O.D. of the samples to the standard curve. 订购热线:4008898798 021-61725725 QQ:2881505690

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电话:13818158258 Materials provided with the kit Materials provided with the kit 48determinations

96 determinations Storage User manual

1 1 Closure plate membrane

2 2 Sealed bags

1 1 Microelisa stripplate

1 1 2-8℃ Standard 0.3ml*

6 bottle 0.3ml*

6 bottle 2-8℃ HRP-Conjugate reagent 5ml*

1 bottle 10ml*

1 bottle 2-8℃ Sample diluent 3ml*

1 bottle 6ml*

1 bottle 2-8℃ Chromogen Solution A 3ml*

1 bottle 6ml*

1 bottle 2-8℃ Chromogen Solution B 3ml*

1 bottle 6ml*

1 bottle 2-8℃ Stop Solution 3ml*

1 bottle 6ml*

1 bottle 2-8℃ 20*Wash solution 15ml*

1 bottle 25ml*

1 bottle 2-8℃ Note: Standard concentration was followed by:

40、

20、

10、

5、2.

5、0 μg/mL. Specimen requirements 1. serum- coagulation at room temperature 10-20 mins, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it. 4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached

1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 5. Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly 订购热线:4008898798 021-61725725 QQ:2881505690

2881505696 监督

电话:13818158258 frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant. 6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can'

t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles. 7. Can'

t detect the sample which contain NaN3, because NaN3 inhibits HRP active. Assay procedure 1. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well. 2.add sample:Set blank wells separately (blank comparison wells don'

t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dil........