PDF《系统发育分析》

动物学杂志 Chinese Journal of Zoology 2018, 53(2):

207 ~

219 克氏光唇鱼线粒体基因组测定及光唇鱼属的 系统发育分析 杨杨 宋小晶 唐文乔? 张亚 海洋动物系统分类与进化上海高校重点实验室,水产科学国家级实验教学示范中心,上海海洋大学 上海

201306 摘要:根据侧条光唇鱼(Acrossocheilus parallens)线粒体基因(mtDNA)序列设计引物,采用引物步 移和 PCR 扩增产物测序,获得了克氏光唇鱼(A.

kreyenbergii)的mtDNA 全序列.结构分析表明,克 氏光唇鱼 mtDNA 为首尾闭合的环状基因,全长

16 596 bp,编码

37 个基因,包括

13 个蛋白编码基因、

22 个tRNA 基因、2 个rRNA 基因和两段非编码区(D-loop 和轻链复制起点 OL) ,碱基组成具有明显 的A+T偏好和反 G 偏倚现象.13 个蛋白编码基因中,除COⅠ的起始密码子是 GTG,其余基因的起 始密码子均为 ATG;

终止密码子包括完全的终止密码子 TAA(38.46%)和TAG(7.69%) ,不完全的 终止密码子 TA(15.38%)和T(38.46%) .在D-loop 区的

811 ~

837 bp 区间发现了一段 TA 短串联 重复序列.从蛋白编码基因所包含的信息量、变异位点和变异率看,ND

5、ND

4、COⅠ和ND2 最适 合作为光唇鱼属种间系统发育分析的分子标记. 采用贝叶斯法利用

13 个蛋白编码基因所构建的系统发 育树显示,光唇鱼属和白甲鱼属(Onychostoma)的24 种鱼类各自没有聚为单系群,相互间不能明确 区分. 关键词:克氏光唇鱼;

线粒体基因组;

系统发育;

光唇鱼属 中图分类号:Q959 文献标识码:A 文章编号:0250-3263(2018)02-207-13 Complete Mitochondrial Genome of Acrossocheilus kreyenbergii, with Phylogenetic Analysis of Genus Acrossocheilus YANG Yang SONG Xiao-Jing TANG Wen-Qiao* ZHANG Ya Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China Abstract: According to the existing mitochondrial gene sequence of Acrossocheilus parallens, primers were designed, and by using the method of primer walking and PCR amplification, mitochondrial genome sequence of A. kreyenbergii was obtained, and its structure was analyzed. The results showed that the complete mitochondrial genome of A. kreyenbergii was a circular genome,

16 596 bp in length, including

13 基金项目 国家科技基础性工作专项(No. 2015FY110200) ;

? 通讯作者,E-mail: [email protected];

第一作者介绍 杨杨,女,硕士研究生;

研究方向:鱼类分子进化;

E-mail: [email protected]. 收稿日期:2017-10-23,修回日期:2017-12-25 DOI: 10.13859/j.cjz.201802006 ・208・ 动物学杂志 Chinese Journal of Zoology

53 卷protein coding genes,

22 tRNA genes,

2 rRNA genes and two non-coding regions (D-loop and origin of light strand replication, OL). The base composition showed two features, the negative bias for G and AT bias. All protein-coding genes started with ATG as an initiation codon except COⅠ, which used GTG. Two complete termination codons were TAA (38.46%) and TAG (7.69%), and two incomplete stop codons were TA (15.38%) and T (38.64%). A control region of

941 bp in length between tRNAPro and tRNAPhe, a short tandem repeat TA, was found in the

811837 bp intervals. From the information contained in the protein coding gene, the mutation site and the mutation rate of view, ND5, ND4, COⅠ and ND2 were the most suitable molecular markers for interspecific phylogenetic analysis of Acrossocheilus. The phylogenetic tree constructed by Bayesian method for