PDF《油茶果糖 - 1,》

第49 卷第11 期2013年11 月林业科学SCIENTIA SILVAE SINICAE Vol.

49, No.

11 Nov. ,2

0 1

3 doi:10.11707 /j.1001- 7488.20131123 收稿日期:

2013 -

04 - 03;

修回日期:

2013 -

06 - 29. 基金项目: 国家自然科学基金项目(31070603) ;

中南林业科技大学青年基金重点项目( QJ2011008A) . * 谭晓风为通讯作者. 油茶果糖 - 1,

6 - 二磷酸醛缩酶基因(CoFBA4)的分子特征与表达分析 * 曾艳玲 谭晓风 蒋瑶刘敏王建勇 周俊琴 ( 中南林业科技大学 森林培养与保护教育部重点实验室 经济林育种与栽培国家林业局重点实验室 长沙 410004) 关键词: 油茶;

果糖 - 1,

6 - 二磷酸醛缩酶;

基因克隆;

基因表达;

亚细胞定位 中图分类号: S718.

46 文献标识码: A 文章编号:

1001 - 7488(2013)11 -

0164 -

07 Molecular Characterization and Expression Analysis of Fructose- 1, 6- Diphosphate Aldolase Gene (CoFBA4) from Camellia oleifera Zeng Yanling Tan Xiaofeng Jiang Yao Liu Min Wang Jianyong Zhou Junqin ( Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education Key Laboratory of Non-Wood Forest Products of State Forestry Administration Central South University of Forestry and Technology Changsha 410004) Abstract: Fructose- 1, 6-diphosphate aldolase (FBA) in plants is not only one of the key regulatory enzyme in glycolysis pathway but also provide substrate source for two key components of oil synthesis. In this paper,a new FBA full-length cDNA of Camellia oleifera seed was isolated and cloned by RACE technology,and was named CoFBA4 ( GenBank number: JX914590). CoFBA4 had an open reading frame with

1 185 base pairs encoding

394 amino acids. Sequence analysis showed that CoFBA4 had nearest genetic distance with FBA of Arabidopsis thaliana,and belonged to subfamily A. CoFBA4 contained glycosylation and fructose- 1, 6-diphosphate aldolase activity site,but had no transmembrane domain. CoFBA4 belonged to hydrophilic protein. Subcellular localization analysis showed that CoFBA4 acted out of cell. There was a positive relationship between expression abundance of CoFBA4 gene and oil yield from different superior clones during the same maturity period. Key words: Camellia oleifera;

fructose- 1, 6-diphosphate aldolase;

gene cloning;

gene expression;

subcellular localization 果糖-1,6 - 二磷酸醛缩酶(fructose- 1, 6- diphosphate aldolase, FBA, EC4. 1. 2. 13), 简称 醛缩酶, 是糖酵解代谢途径中第

4 步关键酶, 催化果糖 - 1,

6 - 二磷酸( Fru- 1, 6-BP) 可逆地裂解为磷酸二羟 丙酮( DHAP) 和3-磷酸甘油醛(G- 3-P) ( Rutter, 1964).其中,DHAP 经甘油磷酸脱氢酶的催化, 还 原生成

3 - 磷酸甘油, 然后在脂酰转移酶的作用下 生成磷脂酸, 再经磷脂酸磷酸酶水解, 形成油脂合成 必需的酯化甘油骨架( Vigeolas et al.,2007);

G- 3-P 则经过多步反应最终形成丙酮酸, 产生乙酰辅酶 A, 经乙酰辅酶 A 羧化酶作用形成脂肪酸合成的底物 丙二酸单酰辅酶 A, 经过一系列聚合反应形成油脂 合成的另一必需元素脂肪酸(Thelen et al.,2002). 油茶(Camellia oleifera)是我国大力推广的优良 食用油料树种, 但产油率低是制约油茶产业快速发 展的主要原因.为了从根本上提高油茶产油率, 近 年来关于油茶油脂合成代谢途径调控基因的研究较 多(谭晓风等,2008a;

2008b;

张党权等,2008), 但 是大部分都集中在脂肪酸方面, 对于调控甘油三磷 酸的基因研究甚少.油茶果糖 - 1,