PDF《高精度定量毛细管电泳法同时测定复合维生素 B 片中》

2019 年6月Vol.

37 No.6 June

2019 Chinese Journal of Chromatography 661~665 研究论文 DOI: 10.3724/ SP.J.1123.2018.12040 ?收稿日期:2018?12?28 ?通讯联系人.Tel:(021)38953588,E?mail:lij@ unimicrotech.com.cn(李静);

Tel:(021)60873372,E?mail:xuxu3426@ sina.com(许旭). 高精度定量毛细管电泳法同时测定复合维生素 B 片中 B1 、B2 、B6 、烟酰胺及泛酸钙 胡雯雯1 , 陶建伟1 , 王庆伟1 , 李静2? , 阎超2,3 , 许旭1? (1. 上海应用技术大学化学与环境工程学院, 上海 201418;

2. 上海通微分析技术有限公司, 上海 201203;

3. 上海交通大学药学院, 上海 200240) 摘要:建立了高精度定量毛细管电泳法同时测定复合维生素 B 片中维生素 B1 、B2 、B6 、烟酰胺和泛酸钙的方法. 样 品经乙腈?水(20 ∶80, v/ v)超声提取后,采用全自动高精度定量毛细管电泳仪,以高精度进样阀定量进样,以40 mmol/ L 硼砂?硼酸缓冲液(pH 9? 0)为背景电解质溶液,以工作电压为-10 kV 电泳分离. 维生素 B1 、B2 、B6 、烟酰 胺的检测波长为

280 nm,然后切换至检测波长

210 nm 检测泛酸钙. 结果表明,各组分之间均得到良好分离,峰面 积日内重复性(RSD)为1? 3% ~1? 9%,显著优于普通毛细管电泳. 维生素 B1 、B2 、B6 、烟酰胺及泛酸钙的浓度在各 自线性范围内的相关系数(r)为0?

996 8~0?

999 8,检出限 2? 5~36?

0 mg/ L,平均回收率为 94? 1% ~ 98? 9%. 该法准 确可靠,可用于实际复合维生素 B 片中维生素 B1 、B2 、B6 、烟酰胺和泛酸钙含量的同时测定. 关键词:毛细管电泳;

复合维生素 B 片;

含量测定 中图分类号:O658 文献标识码:A 文章编号:1000?8713(2019)06?0661?05 Simultaneous determination of vitamins B1, B2, B6, niacinamide and calcium pantothenate in compound vitamin B tablets by high performance quantitative capillary electrophoresis HU Wenwen1 , TAO Jianwei1 , WANG Qingwei1 , LI Jing2? , YAN Chao2,3 , XU Xu1? (1. School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China;

2. Unimicro Technologies, Inc., Shanghai 201203, China;

3. Pharmacy School, Shanghai Jiaotong University, Shanghai 200240, China) Abstract: A high performance quantitative capillary electrophoresis method was developed for the simultaneous determination of vitamins B1, B2, B6, nicotinamide, and calcium pantothenate in vitamin B tablets using a quantitative capillary electrophoresis instrument. The samples were extracted ultrasonically with acetonitrile?water (

20 ∶ 80, v / v). The automatic high precision quantitative capillary electrophoresis instrument was used to realize quantitative injection through a

10 nL injection valve. The background electrolyte was selected as

40 mmol/ L sodium borate buffer (pH 9? 0), which was continuously supplied by a microfluidic injection pump. The working voltage was -10 kV. The detection wavelength of vitamins B1, B2, B6, and nicotinamide was selected as

280 nm, which was then changed to

210 nm to detect calcium pantothenate. The result showed good linearities between the peak area and the concentration of vitamins B1 , B2 , B6 , nicotinamide, and calcium pantothenate in the correlation coefficients ( r) range of 0?

996 8-0?

999 8. The limits of detection ( LODs) were in the range of 2?

5 - 36?

0 mg / L. The average recoveries were 94? 1% - 98? 9% with relative standard deviations ( RSDs ) as 1? 3%-1? 9%. The method is precise, reliable, and suitable for the simultaneous determination of 色谱第37 卷vitamins B1, B2, B6, nicotinamide, and calcium pantothenate in a real compound vitamin B tablet. Key words: capillary electrophoresis ( CE );