PDF《M0491S》

Q5? High-Fidelity DNA Polymerase M0491S

100 units 2,000 U/ml Lot:

0041212 RECOMBINANT Store at C20°C Exp: 12/14 Reaction Conditions: 1X Q5 Reaction Buffer, DNA template, 0.

5 μM primers,

200 μM dNTPs (not included), 1X Q5 High GC Enhancer (optional) and

1 unit of Q5 High-Fidelity DNA Polymerase in a total reaction volume of

50 μl. Unit Definition: One unit is defined as the amount of enzyme that will incorporate

10 nmol of dNTP into acid insoluble material in

30 minutes at 74°C. Unit Assay Conditions:

25 mM TAPS-HCl (pH 9.3 @ 25°C),

50 mM KCl,

2 mM MgCl2 , 1?mM?β-mercaptoethanol,

200 μM dNTPs including [3 H]-dTTP and

400 μg/ml activated Calf Thymus DNA. Heat Inactivation: No (see other side) Endonuclease Activity: Incubation of a

50 μl reaction in NEBuffer

2 containing a minimum of 10?units of Q5 High-Fidelity DNA Polymerase with 200?μM dNTPs and

1 μg of supercoiled pUC19 DNA for

4 hours at either 37°C or 72°C results in