PDF《吴田双江古茶树与云南大叶种茶群体品种间的 ISSR 分析_吴田》

Vol.

32 No.

3 Mar.

2012 第32 卷第3期2012 年3月中南林业科技大学学报Journal of Central South University of Forestry &

Technology 收稿日期:2011-12-30 基金项目:国家林业局

948 项目 (2011-4-45);

西南林业大学重点基金 (110908) 和西南林业大学 大型仪器设备共享基金 作者简介:吴田(1980―),女,山东青岛人,副教授,博士,主要从事植物生物技术及分子生物学方面的研究;

E-mail:461257271@ qq.com 通讯作者:蓝增全 (1963―),男,广东梅县人,教授,主要从事生态学和茶学方向研究;

E-mail: [email protected] 双江古茶树与云南大叶种茶群体品种间的 ISSR 分析 吴田1,沈晓进

2 , 李法营

3 , 韦玲长

4 , 蓝增全

3 (1. 西南林业大学 园林学院,云南 昆明 650224;

2. 昆明市农科院生物所,云南 昆明 650034;

3. 西南林业大学 环境科学与工程学院,云南 昆明 650224;

4. 临沧茶叶科学研究所,云南 临沧 677000) 摘要:采用 ISSR 分子标记技术对云南

17 份茶树样品进行了亲缘关系分析.实验结果表明,12 条ISSR 引 物共产生

193 条扩增带,其中多态性条为 100. 0%.供试材料的遗传相似系数变异范围为 0.50 ~ 0.88.通过 UPGMA 法,可将

17 份材料分为

2 个大类,第1大类为 红毛茶 ,第2大类又可分成

2 个亚组,其中组Ⅰ包 括双江古茶树和永德大叶种,而组Ⅱ包括勐库群体品种、勐海大叶种和部分凤庆大叶种.聚类分析结果表明, 双江古茶树与采自永德的茶树亲缘关系最近,而与采自勐海和凤庆大叶种的亲缘关系相对较远. 关键词: 大叶茶;

种质资源;

ISSR 标记 中图分类号:S794.9;

S571.1 文献标志码:A 文章编号:1673-923X(2012)03-0136-05 ISSR analysis between Shuangjiang ancient tea trees and Yunnan big-leaf tea trees WU Tian1 , SHENG Xiao-jin2 , LI Fa-ying3 , WEI Ling-chang4 , LAN Zeng-quan3 (1. College of Horticulture and Gardening, Southwest Forestry University, Kunming 650224, Yunan, China;

2.Biotechnology Research Institute, Kunming Academy of Agriculture Science, Kunming 650034, Yunan, China;

3. Lincang Tea Research Institute, Lincang 677000, Yunan, China;

4. College of Environment Science and Engineering, Southwest Forestry University, Kunming 650224, Yunan, China) Abstract: ISSR molecular markers were used to analyze the genetic polymorphism of

17 Yunnan big-leaf tea resources. The results show that

193 bands were amplified with

12 ISSR primers, and the polymorphic rate were 100.00 %. The genetic similarity of the materials varied between 0.50 and 0.88. The

17 materials were classified into

2 groups with UPGMA method, and the first group involed Hongmao Tea , and the second one included two subgroups. The subgroup Ⅰ included the Shuangjiang ancient tea trees and Yongde big-leaf, and the subgroup Ⅱ included Mengku , the Menghai big-leaf and parts of Fengqing big-leaf. The genetic relationship of the ancient tea trees from Shuangjiang and parts of Fengqing big-leaf tea was the closest, and that was more far relatively with Menghai big-leaf tea. Key words: big-leaf tea;

germplasm resources;

ISSR marker 云南大叶种茶是云南省大叶类茶树品种的总 称,其代表是经国家认定的勐库大叶种、凤庆大 叶种和勐海大叶种.据国家、省、地茶叶学者专 家科考组

2002 年12 月的初步结论,树龄

2 700 多 年的双江自治县勐库野生古茶树群落,是迄今世 界上已发现的海拔最高、分布面积最广、种群密 度最大的野生古茶树群落.其中,1 号大茶树位 于海拔