PDF《黑果枸杞花青素的制备与纯化研究》

第7卷第9期食品安全质量检测学报Vol.

7 No.

9 2016 年9月Journal of Food Safety and Quality Sep. ,

2016 基金项目: 青海省科技厅高新技术研究与发展计划(2015-GX-201A) Fund: Supported by Project of Hi-Tech Research and Development of Qinghai Science and Technology Department (2015-GX-201A) *通讯作者: 王树林, 博士, 教授, 主要研究方向为青藏高原特色食品资源开发.E-mail: [email protected] *Corresponding author: Wang Shu-Lin, Ph.D, Professor, Agriculture and Animal Husbandry College of Qinghai University, Xining 810016, China. E-mail: [email protected] 黑果枸杞花青素的制备与纯化研究 杭园园, 陈虎, 叶英, 秦艳婷, 刘荟萃, 马文婷, 王树林* (青海大学农牧学院, 西宁 810016) 摘要: 目的 以黑果枸杞为原料, 研究黑果枸杞花青素提取的最佳工艺条件及其纯化工艺, 并对纯化效果进 行检验.方法 采用单因素和正交实验, 以乙醇为提取溶剂, 对花青素进行提取, 对提取温度、提取时间、料 液比和乙醇浓度等参数进行优化;

将提取液离心并蒸发浓缩后用大孔树脂进行纯化, 对纯化工艺进行研究, 确 定最佳纯化工艺.结果 确定黑果枸杞花青素的最佳提取工艺为: 以pH3.0 的75%乙醇溶液为提取溶剂, 提取 温度为

40 ℃, 提取时间为

2 h, 料液比为 1:40.在pH3.0, 温度低于

50 ℃, 流速为

1 mL/min 的条件下, 大孔树 脂对黑果枸杞花青素的最大吸附量达 61.21 mg/mL, 花青素回收率为 44.35%, 花青素含量为

118 mg/g, 是纯化 前的 10.91 倍, 通过冷冻干燥洗脱液得到外观呈黑紫色的花青素产品.结论 确定了黑果枸杞花青素乙醇浸提 法的最佳工艺条件, 证明了 AB-8 大孔树脂具有良好的吸附能力和选择吸附性. 关键词: 黑果枸杞;

花青素;

提取;

纯化 Study on extraction and purification of anthocyanin from Lycium ruthenicum Murr HANG Yuan-Yuan, CHEN Hu, YE Ying, QIN Yan-Ting, LIU Hui-Cui, MA Wen-Ting, WANG Shu-Lin* (Agriculture and Animal Husbandry College of Qinghai University, Xining 810016, China) ABSTRACT: Objective To study on the optimum extraction conditions and purification process of anthocyanin from Lycium ruthenicum Murr (LR) by using LR as raw material, and verify the purification effect. Methods Basing on single factor and orthogonal experiments, the anthocyanin was extracted with ethanol, and the extraction parameters were optimized which included extracting temperature, extracting time, ratio of material to liquid and ethanol concentration. Then the extract was purified by macroporous resin after centrifugation and evaporation concentration, and the purification process was also studied so as to determine the optimum purification process. Results The optimum extraction conditions of anthocyanin from LR were as follows: the extraction solvent was 75% ethanol solution(pH3.0), extracting temperature was

40 ℃, extracting time was

2 h, and ratio of material to liquid was 1:40. Under the conditions of pH3.0, the temperature was lower than

50 ℃, and the current velocity was

1 mL/min, the maximum adsorption capacity of anthocyanin by macroporous resin reached 61.21 mg/mL, the percent recovery and content of anthocyanin were 44.35% and

118 mg/g, respectively. Black purple anthocyanin was obtained from elution solution by freeze drying. Conclusion The optimum process of extracting anthocyanin with ethanol from LR is determined, and it is proved that the AB-8 macroporous resins has a good adsorption capacity and 第9期杭园园, 等: 黑果枸杞花青素的制备与纯化研究