PDF《第7卷第 1期》

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3 . Th e Fu n c t i o n o f Po l i o v i r u s

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9 2 A p r o t e i n a e s i n Po l i o ― Va c c l n a r e c o m bi na nt vi r us , W e c on s t r uc t e d t he va ri o us chi mer i c vi r us o f Po l i o - Va c - c l n i a , a n d o b t a i n e d

4 P o l i o - - Va c c l n i a r e c o mb i n a n t v i r u s e s wh i c h c o n t a i n v a t ― i o us f r a g m e nt s o f Po l i o vi r us I t ype m a ho ne y s t r a i n g e ne. The s e f r a g me nt s we r e i n s e r t e d i n t o t h e TK r e g i o n o f v a c c i nl a g e n e . Th e a n t i g e n o f P o l i o v i r u s was e xpr e s s ed i n t he s e

4 r e c o m hl na nt vi r us e s , t he a nt i bo dy a g ai ns t Po l i o vi r us

1 t ype was i nd uc e d i n t he r a bbi t i nfe c t e d by t he s e c hi m e r i c vi r us e s . But , t he e xpr e s s i ve l e ve l o f t he Pol l ov i r us I t ype ant i g e n Wa S i nfl ue nc e d o bvi o us l y wi t h t he 2A pr o t e i na s e. The r e s ul t a bo ut t he s ul t a hl e g r o wt h o f r e c o mbi na nt vi r u s i n va r i o u s c e l l l i ne s s howe d t ha t 2A pr ot e i na s e di d no t p e r f o r m e d e nt i - r e l y i t s r o l e t ha t s howe d i n Po l i ovl r us i n c el

1 . K ey w or dsI Re c o mb i n s n l v i r u s Po l i o vi r u s 2A p r o t e l n a s e Va c c i t t i a vi r us } 维普资讯 http://www.cqvip.com ........