PDF《微波消解-电感耦合等离子体质谱法同时测定 沙棘汁饮料中 18 ...》

第10 卷第1期食品安全质量检测学报Vol.

10 No.

1 2019 年1月Journal of Food Safety and Quality Jan. ,

2019 基金项目: 十三五 国家重点研发计划项目(2017YFF0211002) ,国家自然科学基金项目(21405159) Fund: Supported by the 13th Five-Year National Key Research and Development Program (2017YFF0211002) and National Natural Science Foundation of China (21405159) *通讯作者: 王海燕, 博士, 副研究员, 主要研究方向为食品、化妆品安全检测.E-mail: [email protected] *Corresponding author: WANG Hai-Yan, Ph.D, Associate Professor, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Dongcheng District, Beijing 100050, China. E-mail: [email protected] 微波消解-电感耦合等离子体质谱法同时测定 沙棘汁饮料中

18 种无机元素 董亚蕾, 刘钊, 高文超, 王海燕* , 曹进, 丁宏(中国食品药品检定研究院, 北京 100050) 摘要: 目的 建立微波消解-电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry, ICP-MS)同时测定沙棘汁饮料中

18 种无机元素的方法.方法 采用微波消解法对沙棘汁饮料样品进行处理, 采用 ICP-MS 法测定, 对前处理过程和仪器条件进行了优化, 对方法性能进行考察, 并将该方法用于

4 批市售 沙棘汁样品中

18 种元素含量的检测.结果 该方法线性范围宽, 线性关系良好(r>

0.995), 精密度高,

18 种元 素的加标回收率为 96.4%~107.3%, 相对标准偏差(relative standard deviation, RSD)为0.3%~5.9%.结论 微波 消解-ICP-MS 法前处理过程简单, 分析时间短, 方法快速、准确、可靠, 能够满足沙棘汁中多种元素同时检测 的需要. 关键词: 沙棘汁;

微波消解;

电感耦合等离子体质谱法;

无机元素 Simultaneous determination of

18 kinds of inorganic elements in sea-buckthorn juice by microwave digestion-inductively coupled plasma mass-spectrometry DONG Ya-Lei, LIU Zhao, GAO Wen-Chao, WANG Hai-Yan* , CAO Jin, DING Hong (National Institutes for Food and Drug Control, Beijing 100050, China) ABSTRACT: Objective To establish a method for simultaneous determination of

18 kinds of inorganic elements in sea-buckthorn juice by microwave digestion-inductively coupled plasma-mass spectrometry (ICP-MS). Methods The sea-buckthorn juice beverage samples were processed by microwave digestion method, then determined by ICP-MS, and the pretreatment process and instrument conditions were optimized. The performance of the method was examined, and the method was applied to detect

18 kinds of inorganic elements in

4 batches of commercially available sea-buckthorn juice samples. Results The method had a wide linear range, good linearity (r>

0.995), high precision, the recoveries of

18 kinds of inorganic elements were 96.4%-107.3%, and the relative standard deviations (RSDs) were 0.3%-5.9%. Conclusion This method has a simple pretreatment process and a short analysis time. It is fast, accurate and reliable, and is suitable for simultaneous determination of various elements in sea-buckthorn juice. KEY WORDS: sea-buckthorn juice;

microwave digestion;

inductively coupling plasma mass spectrometry;

inorganic element

26 食品安全质量检测学报第10 卷1引言沙棘又名醋柳、酸刺等, 在华北、西北、东北及西南 地区多有分布, 是一种新兴的小浆果类树种[1] .沙棘为药 食同源植物,

1977 年被列于《中华人民共和国药典》[2] , 是 古代蒙医、藏医常用药材.长期以来人们重视沙棘果实中 沙棘油的开发[3] , 近年来沙棘汁的开发逐渐引起重视.研 究表明沙棘汁中富含维生素、黄酮、总酚、糖、蛋白质等 成分[4] , 体外实验证明沙棘汁具有很好的自由基清除能力, 对羟自由基致 DNA 的氧化损伤有很强的保护作用, 具有 抗氧化活性[5] .Beata 等[6] 研究表明沙棘中含有对治疗心脑 血管疾病有益的生物活性化合物, 并具有抗肿瘤活性[7] . 随着人们对沙棘汁保健功能的认识, 沙棘汁逐渐被开发, 成为一种天然、绿色、保健、营养的新型果蔬饮料, 得到 人们的青睐. 沙棘汁中含有多种对机体有重大意义的元素, 测定 其中无机元素的含量有助于人们全面了解沙棘汁的保健功 效.目前, 用于测定沙棘汁中元素的方法主要有原子吸收 法、原子荧光法和电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry, ICP-MS).张崇玉等[8] 分 别采用火焰原子吸收法和石墨炉原子吸收法测定了沙棘原 汁中