PDF《欧美杨再生体系的建立》

中国农学通报 2017,33(4):48-53 Chinese Agricultural Science Bulletin 欧美杨再生体系的建立 张瑞芝, 王峰, 李丹蕾, 陈俏丽, 牛春阳, 零雅茗 (东北林业大学, 哈尔滨 150040) 摘要: 黑杨派的杂交后代欧美杨(Populus nigra* P.

deltoides)在杨树叶锈病研究领域具有重要价值.本 研究以叶柄、 叶片和茎段作为外植体进行组织培养, 构建欧美杨再生体系.分别优化了初代培养、 继代 培养、 抽茎培养和生根培养的培养条件.正交试验结果表明, 最优初代培养培养基配方: MS+ 0.5 mg/L 6-BA+ 0.05 mg/L NAA.最优继代培养培养基配方: MS+ 0.5 mg/L 6-BA+ 0.1 mg/L NAA.最优抽茎培 养培养基配方: MS+ 0.1 mg/L 6-BA+ 0.05 mg/L NAA.最优生根培养培养基配方: 1/2MS+ 0.25 mg/L NAA+20 g/L 蔗糖.以上培养基培养分别得到叶柄分化率为 86.7%, 不定芽诱导率为 96.7%, 抽茎率为 91.7%, 生根率为85%.再生苗移栽成活率为91.5%.所获得的欧美杨再生苗扩繁简便, 可用于杨树抗 叶锈病相关基因转化等研究.欧美杨再生体系为揭示欧美杨的感病机理提供物质基础. 关键词: 欧美杨;

组织培养;

再生体系 中图分类号: S792.119, S722.3+7 文献标志码: A 论文编号: casb16060123 Establishment of Populus nigra* P. deltoides Regeneration System Zhang Ruizhi, Wang Feng, Li Danlei, Chen Qiaoli, Niu Chunyang, Ling Yaming (Northeast Forestry University, Harbin 150040) Abstract: The Populus nigra* P. deltoides, the hybrid offspring of Aigeiros, is useful in the pathology of poplar against the infection of rust. The petiole, leaves and stem segments were cultured as explants to induce seedling. Optimal conditions of the regeneration system were determined for the primary culture, subculture, stem elongation culture and rooting culture. While the best primary culture medium (MS+ 0.5 mg/L 6-BA+ 0.05 mg/L NAA) was used, 86.7% of petioles transformed to seedlings. Further more, an adventitious bud induction rate of 96.7% was got when the subculture medium (MS+ 0.5 mg/L 6-BA+ 0.1 mg/L NAA) was adopted. A stem elongation rate of 91.7% was got when the stem culture medium (MS + 0.1 mg/L 6- BA + 0.05 mg/L NAA) was used. While the root media (1/2MS+ 0.25 mg/L NAA+20 g/L) was used, 85% of the seedlings were rooted. The survival rate of the regenerated seedlings was 91.5% . The Populus nigra * P. deltoides regeneration system could be rapidly propagated and used in gene inheritance and transformation. It also could provide material basis for the study of poplar susceptibility/resistance to the rust fungi. Key words: Populus nigra* P. deltoides;

tissue culture;

regeneration system

0 引言 黑杨派是世界上栽培面积最广的杨树品种, 在林 木生产中具有重要的经济地位[1] .欧美杨(Populus nigra* P. deltoides)是黑杨派杂交树种, 既可用作行道 树种, 也可作为园林绿化树种.该树种对生长环境的 要求较低, 可大范围种植, 是三北防护林的重要树种. 基金项目: 国家自然科学基金项目 miR159介导的PndMYB转录调控在欧美杨-落叶松-杨栅锈菌互作中的分子机制研究 (31500524);

中央高校基本 科研业务费专项资金项目 基于RNA-Seq策略研究杨树抗杨褐盘二孢菌(Marssonina brunnea)侵染分子机理 (2572015EY02, 2572015CA09). 第一作者简介: 张瑞芝, 女, 1993年出生, 甘肃张掖人, 硕士研究生, 研究方向: 森林病理学.通信地址:

150040 黑龙江省哈尔滨市和兴路26号 东北林 业大学林学院, Tel: 0451-82190232, E-mail: [email protected]. 通讯作者: 李丹蕾, 女, 1980年出生, 云南普洱人, 副教授, 博士, 研究方向: 森林保护学.通信地址: